Idioma:

Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors

Kawaguchi, Takamasa ; Cho, Dooseon ; Hayashi, Masafumi ; Tsukiyama, Tomoyuki ; Kimura, Koji ; Matsuyama, Shuichi ; Minami, Naojiro ; Yamada, Masayasu ; Imai, Hiroshi Knott, Jason Glenn

PloS one, 2016-12, Vol.11 (12), p.e0167550-e0167550 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors
Autor: Kawaguchi, Takamasa ; Cho, Dooseon ; Hayashi, Masafumi ; Tsukiyama, Tomoyuki ; Kimura, Koji ; Matsuyama, Shuichi ; Minami, Naojiro ; Yamada, Masayasu ; Imai, Hiroshi
Knott, Jason Glenn
Assuntos: Amnion ; Amnion - cytology ; Amnion - drug effects ; Amnion - metabolism ; Amniotic cells ; Animals ; Antibiotics ; Beef cattle ; Biology ; Biology and Life Sciences ; Biomarkers - metabolism ; Biotechnology ; c-Myc protein ; Cattle ; CDX2 protein ; Cell culture ; Cell Line ; Cell lineage ; Cell Lineage - drug effects ; Cell lines ; Colonies ; Comparative analysis ; Cytology ; Differentiation ; Dosage and administration ; Doxycycline ; Doxycycline - pharmacology ; Ectoderm - cytology ; Ectoderm - drug effects ; Ectoderm - metabolism ; Embryos ; Female ; Fibroblasts ; Founder Effect ; Gene Expression ; Genes ; Genetic aspects ; Genetic Vectors - chemistry ; Genetic Vectors - metabolism ; Grasslands ; Growth factors ; Implantation ; Induced Pluripotent Stem Cells - cytology ; Induced Pluripotent Stem Cells - drug effects ; Induced Pluripotent Stem Cells - metabolism ; Interferon ; KLF4 protein ; Kruppel-Like Transcription Factors - genetics ; Kruppel-Like Transcription Factors - metabolism ; Laboratories ; Life sciences ; Livestock ; Mammals ; Morphology ; Myc protein ; Oct-4 protein ; Octamer Transcription Factor-3 - genetics ; Octamer Transcription Factor-3 - metabolism ; Placenta ; Pluripotency ; Pregnancy ; Proto-Oncogene Proteins c-myc - genetics ; Proto-Oncogene Proteins c-myc - metabolism ; Research and Analysis Methods ; SOXB1 Transcription Factors - genetics ; SOXB1 Transcription Factors - metabolism ; Spheroids ; Stem cells ; Transcription factors ; Trophectoderm ; Trophoblasts - cytology ; Trophoblasts - drug effects ; Trophoblasts - metabolism
É parte de: PloS one, 2016-12, Vol.11 (12), p.e0167550-e0167550
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Current address: The Fukui Research Institute, Ono Pharmaceutical Co. Ltd., Fukui, Japan
Conceptualization: TK DC TT HI.Data curation: TK DC.Formal analysis: TK DC MH KK SM.Funding acquisition: KK HI.Investigation: TK DC MH.Methodology: TK DC MH KK SM NM MY.Project administration: KK HI.Resources: KK SM.Supervision: HI.Validation: TK DC TT NM MY HI.Visualization: TK DC.Writing – original draft: TK HI.Writing – review & editing: TK DC HI.
Current address: Laboratory of Reproductive Physiology, Graduate School of Environmental and Life Science, Okayama University, Okayama, Japan
Descrição: Trophectoderm lineage specification is one of the earliest differentiation events in mammalian development. The trophoblast lineage, which is derived from the trophectoderm, mediates implantation and placental formation. However, the processes involved in trophoblastic differentiation and placental formation in cattle remain unclear due to interspecies differences when compared with other model systems and the small repertoire of available trophoblast cell lines. Here, we describe the generation of trophoblast cell lines (biTBCs) from bovine amnion-derived cells (bADCs) using an induced pluripotent stem cell technique. bADCs were introduced with piggyBac vectors containing doxycycline (Dox)-inducible transcription factors (Oct3⁄4(POU5F1), Sox2, Klf4, and c-Myc). Colonies that appeared showed a flattened epithelial-like morphology similar to cobblestones, had a more definite cell boundary between cells, and frequently formed balloon-like spheroids similar to trophoblastic vesicles (TVs). biTBCs were propagated for over 60 passages and expressed trophoblast-related (CDX2, ELF5, ERRβ, and IFN-τ) and pluripotency-related genes (endogenous OCT3/4, SOX2, KLF4, and c-MYC). Furthermore, when biTBCs were induced to differentiate by removing Dox from culture, they formed binucleate cells and began to express pregnancy-related genes (PL, PRP1, and PAG1). This is the first report demonstrating that the induction of pluripotency in bovine amniotic cells allows the generation of trophoblastic cell lines that possess trophoblast stem cell-like characteristics and have the potential to differentiate into the extra-embryonic cell lineage. These cell lines can be a new cell source as a model for studying trophoblast cell lineages and implantation processes in cattle.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors

Kawaguchi, Takamasa ; Cho, Dooseon ; Hayashi, Masafumi ; Tsukiyama, Tomoyuki ; Kimura, Koji ; Matsuyama, Shuichi ; Minami, Naojiro ; Yamada, Masayasu ; Imai, Hiroshi Knott, Jason Glenn

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.