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Nitrogen metabolism-related enzymes in Mesembryanthemum crystallinum after Botrytis cinerea infection

Gajewska, E. ; Surówka, E. ; Kornas, A. ; Kuźniak, E.

Biologia plantarum, 2018-09, Vol.62 (3), p.579-587 [Periódico revisado por pares]

Dordrecht: Springer Netherlands

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Título:
Nitrogen metabolism-related enzymes in Mesembryanthemum crystallinum after Botrytis cinerea infection
Autor: Gajewska, E. ; Surówka, E. ; Kornas, A. ; Kuźniak, E.
Assuntos: Alanine ; Alanine transaminase ; Aspartate aminotransferase ; Biomedical and Life Sciences ; Botrytis cinerea ; Carbon ; Crassulacean acid metabolism ; Crystal structure ; Crystallinity ; Dehydrogenase ; Dehydrogenases ; Enzymatic activity ; Enzymes ; Ferredoxin ; Glutamate dehydrogenase ; Glutamate-ammonia ligase ; Glutamine ; Infections ; Inhibition ; Leaves ; Life Sciences ; Mesembryanthemum crystallinum ; Metabolism ; NAD ; NADH ; Nicotinamide adenine dinucleotide ; Nitrate reductase ; Nitrogen ; Nitrogen metabolism ; Original Paper ; Phosphoenolpyruvate carboxylase ; Photosynthesis ; Plant Sciences
É parte de: Biologia plantarum, 2018-09, Vol.62 (3), p.579-587
Descrição: We compared C 3 and CAM (crassulacean acid metabolism) states in Mesembryanthemum crystallinum , a facultative CAM species, with respect to the involvement of phosphoenolpyruvate carboxylase (PEPC) and nitrogen metabolismrelated enzymes in plant response to Botrytis cinerea infection. The enzyme activities were monitored both in pathogeninoculated 2 nd leaf pair and non-inoculated 3 rd leaf pair. The control activities of most studied enzymes were dependent on the mode of photosynthesis. Compared to C 3 plants, those performing CAM exhibited higher PEPC, nitrate reductase (NR), and deaminating glutamate dehydrogenase (NAD-GDH) activities but lower glutamine synthetase (GS) and alanine aminotransferase (ALT) activities. Regardless of the mode of photosynthetic carbon assimilation, the plants responded to infection with enhancement of PEPC and inhibition of NR activities in the inoculated leaves. Whereas the activity of GS remained unaffected, those of all glutamate-yielding enzymes, namely ferredoxin-dependent glutamate synthase (Fd-GOGAT), aspartate aminotransferase (AST), ALT, and aminating glutamate dehydrogenase (NADHGDH) were altered after infection. However, the time-course and extent of the observed changes differed in C 3 and CAM plants. In general, CAM plants responded to infection with an earlier increase in PEPC and Fd-GOGAT activities as well as later inhibition of NR activity. Contrary to C 3 plants, in those performing CAM the activities of PEPC, Fd-GOGAT, NADH-GDH, and AST in the non-inoculated 3 rd leaf pair were similarly influenced by infection as in leaves directly inoculated with the pathogen. This implies that the local infection induced an alteration of carbon/nitrogen status in healthy upper leaves. This reprogramming resulting from changes in PEPC and nitrogen metabolism-related enzymes was C 3 - and CAM-specific.
Editor: Dordrecht: Springer Netherlands
Idioma: Inglês

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Nitrogen metabolism-related enzymes in Mesembryanthemum crystallinum after Botrytis cinerea infection

Gajewska, E. ; Surówka, E. ; Kornas, A. ; Kuźniak, E.

Dordrecht: Springer Netherlands

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