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Chemiluminescent Determination of Peroxidase with Purpurogallin Carboxylic Acid

SEGAWA, Tadashi ; OOIZUMI, Takahiro ; MATSUBARA, Taichi ; KAMIDATE, Tamio ; WATANABE, Hiroto

Analytical Sciences, 1995/04/10, Vol.11(2), pp.209-212 [Periódico revisado por pares]

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Título:
Chemiluminescent Determination of Peroxidase with Purpurogallin Carboxylic Acid
Autor: SEGAWA, Tadashi ; OOIZUMI, Takahiro ; MATSUBARA, Taichi ; KAMIDATE, Tamio ; WATANABE, Hiroto
Assuntos: Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chemiluminescence ; Enzymes and enzyme inhibitors ; Fundamental and applied biological sciences. Psychology ; Oxidoreductases ; peroxidase ; polyphenol
É parte de: Analytical Sciences, 1995/04/10, Vol.11(2), pp.209-212
Descrição: Purpurogallin carboxylic acid (PurA) was proposed as a highly sensitive chemiluminescent (CL) reagent for the detection of peroxidase. The blank signal of PurA was lower than that of its mother moiety, purpurogallin, thus resulting in a decrease in the detection limit of peroxidase. The calibration curves of horseradish peroxidase and Arthromyces ramosus peroxidase with PurA were linear over the ranges from a detection limit of 20fmol to 5.0pmol and 10fmol to 5.0pmol, respectively. PurA CL was less subject to interference from diverse albumin than was luminol CL.
Editor: Tokyo: The Japan Society for Analytical Chemistry
Idioma: Inglês

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