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Caveolin‐1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells

Barth, K. ; Weinhold, K. ; Guenther, A. ; Young, M. T. ; Schnittler, H. ; Kasper, M.

The FEBS journal, 2007-06, Vol.274 (12), p.3021-3033 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Caveolin‐1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells
Autor: Barth, K. ; Weinhold, K. ; Guenther, A. ; Young, M. T. ; Schnittler, H. ; Kasper, M.
Assuntos: alveolar epithelium ; Anatomy & physiology ; Animals ; Biochemistry ; Caveolin 1 - metabolism ; Caveolin‐1 ; Cell Line ; Cryoelectron Microscopy ; Epithelial Cells - metabolism ; Gene expression ; Lipids ; Lung - cytology ; Lung - metabolism ; Lungs ; Membrane Microdomains - metabolism ; Membrane Microdomains - ultrastructure ; Mice ; Molecular biology ; mouse lung ; P2X7 receptor ; Proteins ; Pulmonary Alveoli - cytology ; Pulmonary Alveoli - metabolism ; Receptors, Purinergic P2 - metabolism ; Receptors, Purinergic P2X7 ; Respiratory Mucosa - cytology ; Respiratory Mucosa - metabolism ; Rodents
É parte de: The FEBS journal, 2007-06, Vol.274 (12), p.3021-3033
Notas: These authors contributed equally to this work
ObjectType-Article-1
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Descrição: The P2X7 receptor has recently been described as a marker for lung alveolar epithelial type I cells. Here, we demonstrate both the expression of P2X7 protein and its partition into lipid rafts in the mouse lung alveolar epithelial cell line E10. A significant degree of colocalization was observed between P2X7 and the raft marker protein Caveolin‐1; also, P2X7 protein was associated with caveolae. A marked reduction in P2X7 immunoreactivity was observed in lung sections prepared from Caveolin‐1‐knockout mice, indicating that Caveolin‐1 expression was required for full expression of P2X7 protein. Indeed, suppression of Caveolin‐1 protein expression in E10 cells using short hairpin RNAs resulted in a large reduction in P2X7 protein expression. Our data demonstrate a potential interaction between P2X7 protein and Caveolin‐1 in lipid rafts, and provide a basis for further functional and biochemical studies to probe the physiologic significance of this interaction.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Caveolin‐1 influences P2X7 receptor expression and localization in mouse lung alveolar epithelial cells

Barth, K. ; Weinhold, K. ; Guenther, A. ; Young, M. T. ; Schnittler, H. ; Kasper, M.

Oxford, UK: Blackwell Publishing Ltd

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