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Cresyl violet: a superior fluorescent lysosomal marker

Ostrowski, Philip P. ; Fairn, Gregory D. ; Grinstein, Sergio ; Johnson, Danielle E.

Traffic (Copenhagen, Denmark), 2016-12, Vol.17 (12), p.1313-1321 [Periódico revisado por pares]

Former Munksgaard: John Wiley & Sons A/S

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Título:
Cresyl violet: a superior fluorescent lysosomal marker
Autor: Ostrowski, Philip P. ; Fairn, Gregory D. ; Grinstein, Sergio ; Johnson, Danielle E.
Assuntos: acidotropic ; Animals ; Benzoxazines - chemistry ; Benzoxazines - metabolism ; Benzoxazines - toxicity ; cresyl violet ; Dogs ; Fluorescent Dyes - chemistry ; Fluorescent Dyes - metabolism ; Fluorescent Dyes - toxicity ; HeLa Cells ; Humans ; Hydrogen-Ion Concentration ; lysosome ; Lysosomes - chemistry ; Lysosomes - metabolism ; Lysosomes - ultrastructure ; LysoTracker ; Madin Darby Canine Kidney Cells ; Mice ; Microscopy, Confocal ; Microscopy, Fluorescence ; Spectrometry, Fluorescence
É parte de: Traffic (Copenhagen, Denmark), 2016-12, Vol.17 (12), p.1313-1321
Descrição: We have characterized cresyl violet as a membrane‐permeant fluorophore that localizes to lysosomes and acidic vacuoles of budding yeast, Drosophila, human, murine and canine cells. An acidotropic weak base, cresyl violet is shown to be virtually insensitive to physiological alkali and divalent cations. Because of its unique spectral properties, it can be used in combination with green, red and far‐red fluorophores, is less susceptible to photobleaching than alternative acidotropic probes, and does not undergo photoconversion. At concentrations that yield bright labeling of acidic compartments, cresyl violet does not alter the organellar pH nor does it affect the buffering capacity. Its affordability, together with its chemical and spectral properties, make cresyl violet a superior lysosomal marker devoid of many of the negative characteristics associated with other lysosomal probes. We have identified cresyl violet as a superior fluorescent lysosomal marker. This red‐shifted fluorophore accumulates selectively in lysosomes in a pH‐dependent manner. Furthermore, we find that cresyl violet is remarkably photostable and does not photoconvert to other fluorescent species, unlike other widely used lysosomal probes. Cresyl violet does not alter the lysosomal buffering capacity, membrane integrity or luminal pH, it is widely available, and costs ≈30 000 times less than competing probes.
Editor: Former Munksgaard: John Wiley & Sons A/S
Idioma: Inglês

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Cresyl violet: a superior fluorescent lysosomal marker

Ostrowski, Philip P. ; Fairn, Gregory D. ; Grinstein, Sergio ; Johnson, Danielle E.

Former Munksgaard: John Wiley & Sons A/S

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