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Quantitative Analysis of Therapeutic Drugs in Dried Blood Spot Samples by Paper Spray Mass Spectrometry: An Avenue to Therapeutic Drug Monitoring

Manicke, Nicholas Edward ; Abu-Rabie, Paul ; Spooner, Neil ; Ouyang, Zheng ; Cooks, R. Graham

Journal of the American Society for Mass Spectrometry, 2011-09, Vol.22 (9), p.1501-1507 [Periódico revisado por pares]

New York: Springer-Verlag

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Título:
Quantitative Analysis of Therapeutic Drugs in Dried Blood Spot Samples by Paper Spray Mass Spectrometry: An Avenue to Therapeutic Drug Monitoring
Autor: Manicke, Nicholas Edward ; Abu-Rabie, Paul ; Spooner, Neil ; Ouyang, Zheng ; Cooks, R. Graham
Assuntos: Analytical Chemistry ; Animals ; Bioinformatics ; Biological and medical sciences ; Biotechnology ; Blood ; Blood Specimen Collection - methods ; Calibration ; Cattle ; Chemistry ; Chemistry and Materials Science ; Drug Monitoring - methods ; Drugs ; General pharmacology ; Humans ; Investigative techniques, diagnostic techniques (general aspects) ; Ions ; Least-Squares Analysis ; Mass spectrometry ; Mass Spectrometry - methods ; Medical sciences ; Miscellaneous ; Miscellaneous. Technology ; Monitoring ; Organic Chemistry ; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques ; Pharmaceutical Preparations - analysis ; Pharmacology. Drug treatments ; Proteomics ; Quantitative analysis ; Reproducibility of Results ; Research Article ; Scientific imaging ; Sensitivity and Specificity ; Spectroscopy ; Therapeutic drug monitoring
É parte de: Journal of the American Society for Mass Spectrometry, 2011-09, Vol.22 (9), p.1501-1507
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A method is presented for the direct quantitative analysis of therapeutic drugs from dried blood spot samples by mass spectrometry. The method, paper spray mass spectrometry, generates gas phase ions directly from the blood card paper used to store dried blood samples without the need for complex sample preparation and separation; the entire time for preparation and analysis of blood samples is around 30 s. Limits of detection were investigated for a chemically diverse set of some 15 therapeutic drugs; hydrophobic and weakly basic drugs, such as sunitinib, citalopram, and verapamil, were found to be routinely detectable at approximately 1 ng/mL. Samples were prepared by addition of the drug to whole blood. Drug concentrations were measured quantitatively over several orders of magnitude, with accuracies within 10% of the expected value and relative standard deviation (RSD) of around 10% by prespotting an internal standard solution onto the paper prior to application of the blood sample. We have demonstrated that paper spray mass spectrometry can be used to quantitatively measure drug concentrations over the entire therapeutic range for a wide variety of drugs. The high quality analytical data obtained indicate that the technique may be a viable option for therapeutic drug monitoring.
Editor: New York: Springer-Verlag
Idioma: Inglês

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Quantitative Analysis of Therapeutic Drugs in Dried Blood Spot Samples by Paper Spray Mass Spectrometry: An Avenue to Therapeutic Drug Monitoring

Manicke, Nicholas Edward ; Abu-Rabie, Paul ; Spooner, Neil ; Ouyang, Zheng ; Cooks, R. Graham

New York: Springer-Verlag

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