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A minimally-edited mouse model for infection with multiple SARS-CoV-2 strains

Nakandakari-Higa, Sandra ; Parsa, Roham ; Reis, Bernardo S ; de Carvalho, Renan V H ; Mesin, Luka ; Hoffmann, Hans-Heinrich ; Bortolatto, Juliana ; Muramatsu, Hiromi ; Lin, Paulo J C ; Bilate, Angelina M ; Rice, Charles M ; Pardi, Norbert ; Mucida, Daniel ; Victora, Gabriel D ; Canesso, Maria Cecilia C

Frontiers in immunology, 2022-11, Vol.13, p.1007080-1007080 [Periódico revisado por pares]

Switzerland: Frontiers Media S.A

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Título:
A minimally-edited mouse model for infection with multiple SARS-CoV-2 strains
Autor: Nakandakari-Higa, Sandra ; Parsa, Roham ; Reis, Bernardo S ; de Carvalho, Renan V H ; Mesin, Luka ; Hoffmann, Hans-Heinrich ; Bortolatto, Juliana ; Muramatsu, Hiromi ; Lin, Paulo J C ; Bilate, Angelina M ; Rice, Charles M ; Pardi, Norbert ; Mucida, Daniel ; Victora, Gabriel D ; Canesso, Maria Cecilia C
Assuntos: Angiotensin-Converting Enzyme 2 - genetics ; Animals ; COVID-19 ; Disease Models, Animal ; Humans ; Immunology ; lung inflammation ; memory B cells ; Mice ; mouse ACE2 ; mRNA vaccine ; Pandemics ; SARS-CoV-2
É parte de: Frontiers in immunology, 2022-11, Vol.13, p.1007080-1007080
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Edited by: Andrea Baragetti, University of Milan, Italy
These authors have contributed equally to this work
This article was submitted to Inflammation, a section of the journal Frontiers in Immunology
Reviewed by: Yanmin Wan, Fudan University, China; Sreelekshmy Mohandas, Indian Council of Medical Research (ICMR), India
Descrição: Efficient mouse models to study SARS-CoV-2 infection are critical for the development and assessment of vaccines and therapeutic approaches to mitigate the current pandemic and prevent reemergence of COVID-19. While the first generation of mouse models allowed SARS-CoV-2 infection and pathogenesis, they relied on ectopic expression and non-physiological levels of human angiotensin-converting enzyme 2 (hACE2). Here we generated a mouse model carrying the minimal set of modifications necessary for productive infection with multiple strains of SARS-CoV-2. Substitution of only three amino acids in the otherwise native mouse locus ( or ™), was sufficient to render mice susceptible to both SARS-CoV-2 strains USA-WA1/2020 and B.1.1.529 (Omicron). Infected ™ mice exhibited weight loss and lung damage and inflammation, similar to COVID-19 patients. Previous exposure to USA-WA1/2020 or mRNA vaccination generated memory B cells that participated in plasmablast responses during breakthrough B.1.1.529 infection. Thus, the ™ mouse replicates human disease after SARS-CoV-2 infection and provides a tool to study immune responses to sequential infections in mice.
Editor: Switzerland: Frontiers Media S.A
Idioma: Inglês

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A minimally-edited mouse model for infection with multiple SARS-CoV-2 strains

Switzerland: Frontiers Media S.A

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