skip to main content
Idioma:

Knockout of E2F1 enhances the polarization of M2 phenotype macrophages to accelerate the wound healing process

Xiao, Hui ; Wu, Yi‐Ping ; Yang, Chang‐Chun ; Yi, Zhen ; Zeng, Ning ; Xu, Yi ; Zeng, Hong ; Deng, Pei ; Zhang, Qi ; Wu, Min

The Kaohsiung journal of medical sciences, 2020-09, Vol.36 (9), p.692-698 [Periódico revisado por pares]

BP, Asia: Wiley Publishing Asia Pty Ltd

Texto completo disponível

Citações Citado por
  • Título:
    Knockout of E2F1 enhances the polarization of M2 phenotype macrophages to accelerate the wound healing process
  • Autor: Xiao, Hui ; Wu, Yi‐Ping ; Yang, Chang‐Chun ; Yi, Zhen ; Zeng, Ning ; Xu, Yi ; Zeng, Hong ; Deng, Pei ; Zhang, Qi ; Wu, Min
  • Assuntos: Antibodies ; Care and treatment ; Cell cycle ; E2F1 ; Genetic aspects ; Genotype & phenotype ; Health aspects ; Macrophages ; Phase transitions ; polarization ; PPAR‐γ ; Proteins ; Rodents ; Skin ; Statistical analysis ; Surgery ; Variance analysis ; Wound healing ; Wounds and injuries
  • É parte de: The Kaohsiung journal of medical sciences, 2020-09, Vol.36 (9), p.692-698
  • Notas: Funding information
    Hui Xiao and Yi‐Ping Wu contributed equally to this work.
    The Science and Technology Project of Hubei Province, China, Grant/Award Number: 2018CFB748
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Wound healing is a complex process, which is classically divided into inflammation, proliferation, and remodeling phases. Macrophages play a key role in wound healing, however, whether E2F1 mediates the M1/M2 polarization during the wound healing process is not known. Skin wounds were surgically induced in E2F1−/− mice and their WT littermates. At day 2 and day 7 post‐surgery, the wounded skin tissues including 2 to 3 mm normal skin were obtained. The wounded skin tissues were used for the analyses of immunofluorescence staining (CD68, iNOS, CD206), western blotting (CD68, iNOS, CD206, PPAR‐γ) and Co‐immunoprecipitation (E2F1‐PPAR‐γ interactions). E2F1−/− mice exhibited faster wound healing process. At day 2, the M2 macrophages were remarkably increased in the E2F1−/− mice. Surprisingly, in the border zone of the wound, E2F1−/− mice had also more M2 macrophages and fewer M1 macrophages at day 7 post‐surgery, suggesting a certain degree of polarization amongst the M1 and M2 phenotypes. Co‐IP revealed that E2F1 indeed interacted with PPAR‐γ, meanwhile western blotting and RT‐PCR showed higher expression of PPAR‐γ in the E2F1−/− mice as compared to that in the WT mice. Therefore, the findings suggest that wound healing process could be accelerated with enhanced M2 polarization through increased PPAR‐γ expression in E2F1 knockout mice.
  • Editor: BP, Asia: Wiley Publishing Asia Pty Ltd
  • Idioma: Inglês;Chinês
Links
Voltar para lista de resultados

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.