skip to main content
Idioma:

Agroinfection of sweet potato by vacuum infiltration of an infectious sweepovirus

Bi, Huiping ; Zhang, Peng

Virologica Sinica, 2014-06, Vol.29 (3), p.148-154 [Periódico revisado por pares]

Heidelberg: Springer-Verlag

Texto completo disponível

Citações Citado por
  • Título:
    Agroinfection of sweet potato by vacuum infiltration of an infectious sweepovirus
  • Autor: Bi, Huiping ; Zhang, Peng
  • Assuntos: Begomovirus ; Begomovirus - pathogenicity ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; breeding ; clones ; cultivars ; DNA ; DNA, Viral - genetics ; Genome, Viral - genetics ; Ipomoea ; Ipomoea batatas - virology ; leaf curling ; leaves ; Lycopersicon esculentum ; Medical Microbiology ; Microbial Genetics and Genomics ; Microbiology ; Nicotiana benthamiana ; Oncology ; pathogenicity ; Plant Diseases - virology ; Research Article ; Solanum tuberosum ; sweet potatoes ; synergism ; Tomato yellow leaf curl China virus ; Vacuum ; Virology ; viruses ; 人工感染 ; 农杆菌 ; 双生病毒 ; 感染性克隆 ; 植物利用 ; 病毒DNA ; 真空渗透 ; 红薯
  • É parte de: Virologica Sinica, 2014-06, Vol.29 (3), p.148-154
  • Notas: Sweepovirus is an important monopartite begomovirus that infects plants of the genus Ipomoea worldwide. Development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of studying infectivity in sweet potato. Unlike other begomoviruses, it has proven difficult to infect sweet potato plants with sweepoviruses using infectious clones. A novel sweepovirus, called Sweet potato leaf curl virus-Jiangsu（SPLCV-JS）, was recently identified in China. In addition, the infectivity of the SPLCV-JS clone has been demonstrated in Nicotiana benthamiana. Here we describe the agroinfection of the sweet potato cultivar Xushu 22 with the SPLCV-JS infectious clone using vacuum infiltration. Yellowing symptoms were observed in newly emerged leaves. Molecular analysis confirmed successful inoculation by the detection of viral DNA. A synergistic effect of SPLCV-JS and the heterologous betasatellite DNA-β of Tomato yellow leaf curl China virus isolate Y10（TYLCCNV-Y10） on enhanced symptom severity and viral DNA accumulation was confirmed. The development of a routine agroinoculation system in sweet potato with SPLCV-JS using vacuum infiltration should facilitate the molecular study of sweepovirus in this host and permit the evaluation of virus resistance of sweet potato plants in breeding programs.
    42-1760/Q
    sweepovirus agroinfection vacuum infiltration sweet potato
    http://dx.doi.org/10.1007/s12250-014-3430-1
    ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: Sweepovirus is an important monopartite begomovirus that infects plants of the genus Ipomoea worldwide. Development of artificial infection methods for sweepovirus using agroinoculation is a highly efficient means of studying infectivity in sweet potato. Unlike other begomoviruses, it has proven difficult to infect sweet potato plants with sweepoviruses using infectious clones. A novel sweepovirus, called Sweet potato leaf curl virus-Jiangsu（SPLCV-JS）, was recently identified in China. In addition, the infectivity of the SPLCV-JS clone has been demonstrated in Nicotiana benthamiana. Here we describe the agroinfection of the sweet potato cultivar Xushu 22 with the SPLCV-JS infectious clone using vacuum infiltration. Yellowing symptoms were observed in newly emerged leaves. Molecular analysis confirmed successful inoculation by the detection of viral DNA. A synergistic effect of SPLCV-JS and the heterologous betasatellite DNA-β of Tomato yellow leaf curl China virus isolate Y10（TYLCCNV-Y10） on enhanced symptom severity and viral DNA accumulation was confirmed. The development of a routine agroinoculation system in sweet potato with SPLCV-JS using vacuum infiltration should facilitate the molecular study of sweepovirus in this host and permit the evaluation of virus resistance of sweet potato plants in breeding programs.
  • Editor: Heidelberg: Springer-Verlag
  • Idioma: Inglês;Chinês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  10 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.