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Arachidonic acid enhances turnover of the dermal skeleton: studies on zebrafish scales

de Vrieze, Erik ; Moren, Mari ; Metz, Juriaan R ; Flik, Gert ; Lie, Kai Kristoffer Winkler, Christoph

PloS one, 2014-02, Vol.9 (2), p.e89347-e89347 [Periódico revisado por pares]

United States: Public Library of Science

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Arachidonic acid enhances turnover of the dermal skeleton: studies on zebrafish scales
Autor: de Vrieze, Erik ; Moren, Mari ; Metz, Juriaan R ; Flik, Gert ; Lie, Kai Kristoffer
Winkler, Christoph
Assuntos: Agriculture ; Amino acids ; Analysis ; Animal Structures - drug effects ; Animal Structures - physiology ; Animal tissues ; Animals ; Aquaculture ; Arachidonic acid ; Arachidonic Acid - pharmacology ; Biocompatibility ; Biology ; Biomedical materials ; Bone density ; Bone growth ; Bone remodeling ; Calcium - metabolism ; Danio rerio ; Dermis - drug effects ; Dermis - physiology ; Dietary supplements ; Dinoprostone - metabolism ; Enzymes ; Fatty acids ; Fish ; Fish nutrition ; Fish oils ; Gene expression ; Gene Expression Profiling ; Mammals ; Matrix metalloproteinase ; Matrix metalloproteinases ; Matrix Metalloproteinases - metabolism ; Metabolism ; Metabolites ; Metalloproteinase ; Mineralization ; Nutrient deficiency ; Nutrition ; Omega 6 fatty acids ; Ontogeny ; Osteoclasts ; Osteogenesis ; Phosphorus - metabolism ; Physiology ; Polyunsaturated fatty acids ; Prostaglandin E2 ; Prostaglandins E ; Regeneration ; Regeneration - drug effects ; Scales ; Seafood ; Skeleton ; Skin ; Studies ; Unsaturated fatty acids ; Vegetable oils ; Wetlands ; Zebrafish ; Zebrafish - physiology
É parte de: PloS one, 2014-02, Vol.9 (2), p.e89347-e89347
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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content type line 23
Conceived and designed the experiments: EDV MM JRM GF KKL. Performed the experiments: EDV JRM KKL. Analyzed the data: EDV MM JRM GF KKL. Contributed reagents/materials/analysis tools: MM GF KKL. Wrote the paper: EDV MM JRM GF KKL.
Competing Interests: The authors have declared that no competing interests exist.
Descrição: In fish nutrition, the ratio between omega-3 and omega-6 poly-unsaturated fatty acids influences skeletal development. Supplementation of fish oils with vegetable oils increases the content of omega-6 fatty acids, such as arachidonic acid in the diet. Arachidonic acid is metabolized by cyclooxygenases to prostaglandin E2, an eicosanoid with effects on bone formation and remodeling. To elucidate effects of poly-unsaturated fatty acids on developing and existing skeletal tissues, zebrafish (Danio rerio) were fed (micro-) diets low and high in arachidonic acid content. Elasmoid scales, dermal skeletal plates, are ideal to study skeletal metabolism in zebrafish and were exploited in the present study. The fatty acid profile resulting from a high arachidonic acid diet induced mild but significant increase in matrix resorption in ontogenetic scales of adult zebrafish. Arachidonic acid affected scale regeneration (following removal of ontogenetic scales): mineral deposition was altered and both gene expression and enzymatic matrix metalloproteinase activity changed towards enhanced osteoclastic activity. Arachidonic acid also clearly stimulates matrix metalloproteinase activity in vitro, which implies that resorptive effects of arachidonic acid are mediated by matrix metalloproteinases. The gene expression profile further suggests that arachidonic acid increases maturation rate of the regenerating scale; in other words, enhances turnover. The zebrafish scale is an excellent model to study how and which fatty acids affect skeletal formation.
Editor: United States: Public Library of Science
Idioma: Inglês

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Arachidonic acid enhances turnover of the dermal skeleton: studies on zebrafish scales

de Vrieze, Erik ; Moren, Mari ; Metz, Juriaan R ; Flik, Gert ; Lie, Kai Kristoffer Winkler, Christoph

United States: Public Library of Science

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