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Aberrant Promoter Methylation of Multiple Genes during Pathogenesis of Bladder Cancer

BRAIT, Mariana ; BEGUM, Shahnaz ; CARVALHO, André L ; DASGUPTA, Santanu ; VETTORE, André L ; CZERNIAK, Bogdan ; CABALLERO, Otavia L ; WESTRA, William H ; SIDRANSKY, David ; OBAIDUL HOQUE, Mohammad

Cancer epidemiology, biomarkers & prevention, 2008-10, Vol.17 (10), p.2786-2794 [Periódico revisado por pares]

Philadelphia, PA: American Association for Cancer Research

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Título:
Aberrant Promoter Methylation of Multiple Genes during Pathogenesis of Bladder Cancer
Autor: BRAIT, Mariana ; BEGUM, Shahnaz ; CARVALHO, André L ; DASGUPTA, Santanu ; VETTORE, André L ; CZERNIAK, Bogdan ; CABALLERO, Otavia L ; WESTRA, William H ; SIDRANSKY, David ; OBAIDUL HOQUE, Mohammad
Assuntos: Aged ; Analysis of Variance ; Biological and medical sciences ; Biomarkers ; Biomarkers, Tumor - genetics ; Bladder Cancer ; Chi-Square Distribution ; DNA Methylation ; Female ; Humans ; Male ; Medical sciences ; Middle Aged ; Nephrology. Urinary tract diseases ; Polymerase Chain Reaction - methods ; Promoter Methylation ; Proportional Hazards Models ; Tumors ; Tumors of the urinary system ; Urinary Bladder Neoplasms - genetics ; Urinary tract. Prostate gland
É parte de: Cancer epidemiology, biomarkers & prevention, 2008-10, Vol.17 (10), p.2786-2794
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Purpose: The aims of our study were to elucidate the role of methylation of a large panel of genes during multistage pathogenesis of bladder cancer and to correlate our findings with patient age and other clinicopathologic features. Experimental Design: We studied the methylation status of 21 genes by quantitative methylation-specific PCR in an evaluation set of 25 tumor and 5 normal samples. Based on methylation frequency in tumors and normals in gene evaluation set, we selected 7 candidate genes and tested an independent set of 93 tumors and 26 normals. The presence or absence of methylation was evaluated for an association with cancer using cross-tabulations and χ 2 or Fisher's exact tests as appropriate. All statistical tests were two-sided. Results: Most primary tumors (89 of 93, 96%) had methylation of one or more genes of independent set; 53 (57%) CCNA1 , 29 (31%) MINT1 , 36 (39%) CRBP , 53 (57%) CCND2 , 66 (71%) PGP9.5 , 60 (65%) CALCA , and 78 (84%) AIM1 . Normal uroepithelium samples from 26 controls revealed no methylation of the CCNA1 and MINT1 genes, whereas methylation of CRBP, CCND2, PGP9.5 , and CALCA was detected at low levels. All the 7 genes in independent set were tightly correlated with each other and 3 of these genes showed increased methylation frequencies in bladder cancer with increasing age. PGP9.5 and AIM1 methylation correlated with primary tumor invasion. Conclusion: Our results indicate that the methylation profile of novel genes in bladder cancers correlates with clinicopathologic features of poor prognosis and is an age-related phenomenon. (Cancer Epidemiol Biomarkers Prev 2008;17(10):2786–94)
Editor: Philadelphia, PA: American Association for Cancer Research
Idioma: Inglês

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Aberrant Promoter Methylation of Multiple Genes during Pathogenesis of Bladder Cancer

BRAIT, Mariana ; BEGUM, Shahnaz ; CARVALHO, André L ; DASGUPTA, Santanu ; VETTORE, André L ; CZERNIAK, Bogdan ; CABALLERO, Otavia L ; WESTRA, William H ; SIDRANSKY, David ; OBAIDUL HOQUE, Mohammad

Philadelphia, PA: American Association for Cancer Research

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