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Standardization and Validation of Fluorescence-Based Quantitative Assay to Study Human Platelet Adhesion to Extracellular-Matrix in a 384-Well Plate

Martins Lima, Augusto ; Saint Auguste, Damian S ; Cuenot, François ; Martins Cavaco, Ana C ; Lachkar, Tom ; Khawand, Cindy Marie Elodie ; Fraga-Silva, Rodrigo A ; Stergiopulos, Nikolaos

International journal of molecular sciences, 2020-09, Vol.21 (18), p.6539 [Periódico revisado por pares]

Switzerland: MDPI AG

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Título:
Standardization and Validation of Fluorescence-Based Quantitative Assay to Study Human Platelet Adhesion to Extracellular-Matrix in a 384-Well Plate
Autor: Martins Lima, Augusto ; Saint Auguste, Damian S ; Cuenot, François ; Martins Cavaco, Ana C ; Lachkar, Tom ; Khawand, Cindy Marie Elodie ; Fraga-Silva, Rodrigo A ; Stergiopulos, Nikolaos
Assuntos: 384-well plate ; Adhesion ; Adhesives ; Assaying ; BCECF-AM ; Blood clots ; Blood platelets ; Blood Platelets - physiology ; Cell adhesion & migration ; Collagen ; Endothelial Cells ; Endothelium, Vascular ; Extracellular matrix ; Extracellular Matrix - physiology ; Fluorescence ; fluorescence-based quantitative assay ; High-throughput screening ; high-throughput screening assay ; Humans ; Immune response ; Immunology ; Leukocytes ; Optical Imaging - methods ; Optical Imaging - standards ; Platelet Activation ; platelet adhesion ; Platelet Adhesiveness - physiology ; Proteins ; Quantitative analysis ; Reference Standards ; Reproducibility of Results ; Standardization ; Thrombosis ; Vascular diseases ; Vitamin E
É parte de: International journal of molecular sciences, 2020-09, Vol.21 (18), p.6539
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Descrição: Platelets play a crucial role in the immunological response and are involved in the pathological settings of vascular diseases, and their adhesion to the extracellular matrix is important to bring leukocytes close to the endothelial cells and to form and stabilize the thrombus. Currently there are several methods to study platelet adhesion; however, the optimal parameters to perform the assay vary among studies, which hinders their comparison and reproducibility. Here, a standardization and validation of a fluorescence-based quantitative adhesion assay to study platelet-ECM interaction in a high-throughput screening format is proposed. Our study confirms that fluorescence-based quantitative assays can be effectively used to detect platelet adhesion, in which BCECF-AM presents the highest sensitivity in comparison to other dyes.
Editor: Switzerland: MDPI AG
Idioma: Inglês

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Standardization and Validation of Fluorescence-Based Quantitative Assay to Study Human Platelet Adhesion to Extracellular-Matrix in a 384-Well Plate

Martins Lima, Augusto ; Saint Auguste, Damian S ; Cuenot, François ; Martins Cavaco, Ana C ; Lachkar, Tom ; Khawand, Cindy Marie Elodie ; Fraga-Silva, Rodrigo A ; Stergiopulos, Nikolaos

Switzerland: MDPI AG

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