Idioma:

Super-resolution imaging reveals the evolution of higher-order chromatin folding in early carcinogenesis

Xu, Jianquan ; Ma, Hongqiang ; Ma, Hongbin ; Jiang, Wei ; Mela, Christopher A ; Duan, Meihan ; Zhao, Shimei ; Gao, Chenxi ; Hahm, Eun-Ryeong ; Lardo, Santana M ; Troy, Kris ; Sun, Ming ; Pai, Reet ; Stolz, Donna B ; Zhang, Lin ; Singh, Shivendra ; Brand, Randall E ; Hartman, Douglas J ; Hu, Jing ; Hainer, Sarah J ; Liu, Yang

Nature communications, 2020-04, Vol.11 (1), p.1899-17, Article 1899 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Super-resolution imaging reveals the evolution of higher-order chromatin folding in early carcinogenesis
Autor: Xu, Jianquan ; Ma, Hongqiang ; Ma, Hongbin ; Jiang, Wei ; Mela, Christopher A ; Duan, Meihan ; Zhao, Shimei ; Gao, Chenxi ; Hahm, Eun-Ryeong ; Lardo, Santana M ; Troy, Kris ; Sun, Ming ; Pai, Reet ; Stolz, Donna B ; Zhang, Lin ; Singh, Shivendra ; Brand, Randall E ; Hartman, Douglas J ; Hu, Jing ; Hainer, Sarah J ; Liu, Yang
Assuntos: Animals ; Biochemical characteristics ; Biochemistry ; Biophysics ; Cancer ; Carcinogenesis ; Carcinogenesis - pathology ; Carcinogens ; Chromatin ; Chromatin - pathology ; Deoxyribonucleic acid ; DNA ; Epigenesis, Genetic ; Epigenetics ; Folding ; Fragmentation ; Genome ; Genomics ; Health risks ; Heterochromatin ; Humans ; Image Processing, Computer-Assisted ; Image resolution ; Male ; Medical imaging ; Mice ; Microscopy ; Microscopy, Fluorescence - methods ; Neoplasms - diagnostic imaging ; Neoplasms - pathology ; Prostatic Neoplasms - diagnostic imaging ; Prostatic Neoplasms - pathology ; Stochasticity ; Transcription ; Transcriptome ; Tumors
É parte de: Nature communications, 2020-04, Vol.11 (1), p.1899-17, Article 1899
Descrição: Genomic DNA is folded into a higher-order structure that regulates transcription and maintains genomic stability. Although progress has been made on understanding biochemical characteristics of epigenetic modifications in cancer, the in-situ higher-order folding of chromatin structure during malignant transformation remains largely unknown. Here, using optimized stochastic optical reconstruction microscopy (STORM) for pathological tissue (PathSTORM), we uncover a gradual decompaction and fragmentation of higher-order chromatin folding throughout all stages of carcinogenesis in multiple tumor types, and prior to tumor formation. Our integrated imaging, genomic, and transcriptomic analyses reveal functional consequences in enhanced transcription activities and impaired genomic stability. We also demonstrate the potential of imaging higher-order chromatin disruption to detect high-risk precursors that cannot be distinguished by conventional pathology. Taken together, our findings reveal gradual decompaction and fragmentation of higher-order chromatin structure as an enabling characteristic in early carcinogenesis to facilitate malignant transformation, which may improve cancer diagnosis, risk stratification, and prevention.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Super-resolution imaging reveals the evolution of higher-order chromatin folding in early carcinogenesis

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.