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Simple, sensitive, specific self-sampling assay secures SARS-CoV-2 antibody signals in sero-prevalence and post-vaccine studies

Khan, Maryam ; Rosadas, Carolina ; Katsanovskaja, Ksenia ; Weber, Isaac D ; Shute, Justin ; Ijaz, Samreen ; Marchesin, Federica ; McClure, Eleanor ; Elias, Salem ; Flower, Barnaby ; Gao, He ; Quinlan, Rachael ; Short, Charlotte ; Rosa, Annachiara ; Roustan, Chloe ; Moshe, Maya ; Taylor, Graham P ; Elliott, Paul ; Cooke, Graham S ; Cherepanov, Peter ; Parker, Eleanor ; McClure, Myra O ; Tedder, Richard S

Scientific reports, 2022-02, Vol.12 (1), p.1885-1885, Article 1885 [Periódico revisado por pares]

England: Nature Publishing Group

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Título:
Simple, sensitive, specific self-sampling assay secures SARS-CoV-2 antibody signals in sero-prevalence and post-vaccine studies
Autor: Khan, Maryam ; Rosadas, Carolina ; Katsanovskaja, Ksenia ; Weber, Isaac D ; Shute, Justin ; Ijaz, Samreen ; Marchesin, Federica ; McClure, Eleanor ; Elias, Salem ; Flower, Barnaby ; Gao, He ; Quinlan, Rachael ; Short, Charlotte ; Rosa, Annachiara ; Roustan, Chloe ; Moshe, Maya ; Taylor, Graham P ; Elliott, Paul ; Cooke, Graham S ; Cherepanov, Peter ; Parker, Eleanor ; McClure, Myra O ; Tedder, Richard S
Assuntos: Antibodies ; Antibodies, Viral - blood ; Biomarkers - blood ; COVID-19 - diagnosis ; COVID-19 - epidemiology ; COVID-19 - immunology ; COVID-19 - virology ; COVID-19 Testing - methods ; COVID-19 Vaccines - immunology ; Dried Blood Spot Testing - methods ; Feasibility Studies ; Female ; Humans ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunoglobulin M ; Immunoglobulin M - blood ; Male ; Medical personnel ; Sampling ; SARS-CoV-2 - immunology ; Sensitivity and Specificity ; Seroepidemiologic Studies ; Serology ; Severe acute respiratory syndrome coronavirus 2 ; Specimen Handling - methods ; Vaccines
É parte de: Scientific reports, 2022-02, Vol.12 (1), p.1885-1885, Article 1885
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: At-home sampling is key to large scale seroprevalence studies. Dried blood spot (DBS) self-sampling removes the need for medical personnel for specimen collection but facilitates specimen referral to an appropriately accredited laboratory for accurate sample analysis. To establish a highly sensitive and specific antibody assay that would facilitate self-sampling for prevalence and vaccine-response studies. Paired sera and DBS eluates collected from 439 sero-positive, 382 sero-negative individuals and DBS from 34 vaccine recipients were assayed by capture ELISAs for IgG and IgM antibody to SARS-CoV-2. IgG and IgM combined on DBS eluates achieved a diagnostic sensitivity of 97.9% (95%CI 96.6 to 99.3) and a specificity of 99.2% (95% CI 98.4 to 100) compared to serum, displaying limits of detection equivalent to 23 and 10 WHO IU/ml, respectively. A strong correlation (r = 0.81) was observed between serum and DBS reactivities. Reactivity remained stable with samples deliberately rendered inadequate, (p = 0.234) and when samples were accidentally damaged or 'invalid'. All vaccine recipients were sero-positive. This assay provides a secure method for self-sampling by DBS with a sensitivity comparable to serum. The feasibility of DBS testing in sero-prevalence studies and in monitoring post-vaccine responses was confirmed, offering a robust and reliable tool for serological monitoring at a population level.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Simple, sensitive, specific self-sampling assay secures SARS-CoV-2 antibody signals in sero-prevalence and post-vaccine studies

England: Nature Publishing Group

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