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Palindromic Molecular Beacon Based Z‑Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection

Zeng, Ruijin ; Luo, Zhongbin ; Su, Lingshan ; Zhang, Lijia ; Tang, Dianping ; Niessner, Reinhard ; Knopp, Dietmar

Analytical chemistry (Washington), 2019-02, Vol.91 (3), p.2447-2454 [Periódico revisado por pares]

United States: American Chemical Society

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  • Título:
    Palindromic Molecular Beacon Based Z‑Scheme BiOCl-Au-CdS Photoelectrochemical Biodetection
  • Autor: Zeng, Ruijin ; Luo, Zhongbin ; Su, Lingshan ; Zhang, Lijia ; Tang, Dianping ; Niessner, Reinhard ; Knopp, Dietmar
  • Assuntos: Aptamers ; Biosensors ; Bismuth ; Cadmium sulfide ; Chemistry ; Deoxyribonucleic acid ; DNA ; Electron transfer ; Fragmentation ; Fragments ; Gold ; Hybridization ; Kanamycin ; Molecular machines ; Nanoparticles ; Nicking endonuclease ; Photoelectric effect ; Photoelectric emission ; Polymerization ; Quantum dots ; Substrates ; Synergistic effect
  • É parte de: Analytical chemistry (Washington), 2019-02, Vol.91 (3), p.2447-2454
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
  • Descrição: This work presented an innovative and rationally engineered palindromic molecular beacon (PMB) based “Z-scheme” photoelectrochemical (PEC) biosensing protocol for the selective screening of kanamycin (Kana) through DNA hybridization-induced conformational conversion. Interestingly, the ingeniously designed PMB integrated the multifunctional elements including recognition region, primer-like palindromic fragment, and polymerization-nicking template. The cosensitized structures consisted of CdS quantum dot functionalized hairpin DNA2 (QD-HP2) and region-selectively deposited gold nanoparticles onto {001} facets of bismuth oxychloride (BiOCl-Au). Compared with BiOCl-Au alone, the attachment of CdS QDs onto BiOCl-Au (i.e., BiOCl-Au-CdS QDs) exhibited evidently enhanced photocurrent intensity thanks to the synergistic effect of Z-scheme BiOCl-Au-CdS QDs. After incubation with target Kana, Kana–aptamer binding could induce the exposure of PMB region for hairpin DNA1 (HP1). The exposed palindromic tails hybridized with each other (like a molecular machine) to consume the substrates (dNTPs) and fuels (enzyme) for the releasing of numerous nick fragments (Nick). The as-generated nick fragments could specifically hybridize with the complementary region of QD-HP2, thus resulting in decreasing photocurrent because of the increasing spatial distance for electron transfer between two-type photosensitizers. Under optimum conditions, the PMB-based sensing system exhibited satisfying photocurrent responses toward target Kana within the working range from 50 to 5000 fM at a low detection limit of 29 fM. Impressively, the concept of a palindromic fragment-mediated primer-free biosensing strategy offers a new avenue for advanced development of efficient and convenient biodetection systems.
  • Editor: United States: American Chemical Society
  • Idioma: Inglês
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