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Orthodontic tooth movement alters cementocyte ultrastructure and cellular cementum proteome signature

Lira dos Santos, Elis J. ; de Almeida, Amanda B. ; Chavez, Michael B. ; Salmon, Cristiane R. ; Mofatto, Luciana S. ; Camara-Souza, Mariana Barbosa ; Tan, Michelle H. ; Kolli, Tamara N. ; Mohamed, Fatma F. ; Chu, Emily Y. ; Novaes, Pedro Duarte ; Santos, Eduardo C.A. ; Kantovitz, Kamila R. ; Foster, Brian L. ; Nociti, Francisco H.

Bone (New York, N.Y.), 2021-12, Vol.153, p.116139-116139, Article 116139 [Periódico revisado por pares]

Elsevier Inc

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  • Título:
    Orthodontic tooth movement alters cementocyte ultrastructure and cellular cementum proteome signature
  • Autor: Lira dos Santos, Elis J. ; de Almeida, Amanda B. ; Chavez, Michael B. ; Salmon, Cristiane R. ; Mofatto, Luciana S. ; Camara-Souza, Mariana Barbosa ; Tan, Michelle H. ; Kolli, Tamara N. ; Mohamed, Fatma F. ; Chu, Emily Y. ; Novaes, Pedro Duarte ; Santos, Eduardo C.A. ; Kantovitz, Kamila R. ; Foster, Brian L. ; Nociti, Francisco H.
  • Assuntos: Cementocytes ; Extracellular matrix ; Mineralized tissue/development ; Orthodontic tooth movement ; Periodontal tissues/periodontium ; Root resorption
  • É parte de: Bone (New York, N.Y.), 2021-12, Vol.153, p.116139-116139, Article 116139
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    content type line 23
    AUTHOR CONTRIBUTIONS
    E.J. Lira dos Santos contributed to conception, design, data acquisition, analysis, and interpretation, and drafted and critically revised the manuscript; C. R. Salmon and A. B. de Almeida contributed to data acquisition, analysis, and interpretation, and critically revised the manuscript; M.B. Chavez contributed to conception, design, data acquisition, analysis, and interpretation, and critically revised the manuscript; L.S. Mofatto contributed to data analysis and interpretation, and critically revised the manuscript; M.B. Camara-Souza, M.H. Tan, T.N. Kolli, E. Y. Chu and F. F. Mohamed contributed to data acquisition, analysis, and interpretation, and critically revised the manuscript; E.A. Santos contributed to conception, design, analysis, and interpretation, and critically revised the manuscript; B.L. Foster contributed to conception, design, data acquisition, analysis, and interpretation, drafted and critically revised the manuscript; F.H. Nociti Jr contributed to conception, design, data acquisition, analysis, and interpretation, and drafted and critically revised the manuscript. All authors gave final approval and agreed to be accountable for all aspects of the work.
  • Descrição: Cementum is a mineralized tissue that covers tooth roots and functions in the periodontal attachment complex. Cementocytes, resident cells of cellular cementum, share many characteristics with osteocytes, are mechanoresponsive cells that direct bone remodeling based on changes in loading. We hypothesized that cementocytes play a key role during orthodontic tooth movement (OTM). To test this hypothesis, we used 8-week-old male Wistar rats in a model of OTM for 2, 7, or 14 days (0.5 N), whereas unloaded contralateral teeth served as controls. Tissue and cell responses were analyzed by high-resolution micro-computed tomography, histology, tartrate-resistant acid phosphatase staining for odontoclasts/osteoclasts, and transmission electron microscopy. In addition, laser capture microdissection was used to collect cellular cementum, and extracted proteins were identified by liquid chromatography coupled to tandem mass spectrometry. The OTM model successfully moved first molars mesially more than 250 μm by 14 days introducing apoptosis in a small number of cementocytes and areas of root resorption on mesial and distal aspects. Cementocytes showed increased nuclear size and proportion of euchromatin suggesting cellular activity. Proteomic analysis identified 168 proteins in cellular cementum with 21 proteins found only in OTM sites and 54 proteins only present in control samples. OTM-down-regulated several extracellular matrix proteins, including decorin, biglycan, asporin, and periostin, localized to cementum and PDL by immunostaining. Furthermore, type IV collagen (COL14A1) was the protein most down-regulated (−45-fold) by OTM and immunolocalized to cells at the cementum-dentin junction. Eleven keratins were significantly increased by OTM, and a pan-keratin antibody indicated keratin localization primarily in epithelial remnants of Hertwig's epithelial root sheath. These experiments provide new insights into biological responses of cementocytes and cellular cementum to OTM.
  • Editor: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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