Idioma:

Complexation and sequestration of BMP-2 from an ECM mimetic hyaluronan gel for improved bone formation

Kisiel, Marta ; Klar, Agnieszka S ; Ventura, Manuela ; Buijs, Jos ; Mafina, Marc-Krystelle ; Cool, Simon M ; Hilborn, Jöns Karamanos, Nikos K

PloS one, 2013-10, Vol.8 (10), p.e78551-e78551 [Periódico revisado por pares]

United States: Public Library of Science

Texto completo disponível

Citações Citado por

Enviar para

Título:
Complexation and sequestration of BMP-2 from an ECM mimetic hyaluronan gel for improved bone formation
Autor: Kisiel, Marta ; Klar, Agnieszka S ; Ventura, Manuela ; Buijs, Jos ; Mafina, Marc-Krystelle ; Cool, Simon M ; Hilborn, Jöns
Karamanos, Nikos K
Assuntos: Adjuvants, Immunologic - chemistry ; Adjuvants, Immunologic - pharmacology ; Alkaline phosphatase ; Animals ; Anticoagulants ; Anticoagulants (Medicine) ; Binding sites ; Biomedical materials ; BMP-2 release ; Bone growth ; Bone morphogenetic protein 2 ; Bone Morphogenetic Protein 2 - chemistry ; Bone Morphogenetic Protein 2 - pharmacology ; Bone morphogenetic proteins ; Bone repair ; Bones ; Cells, Cultured ; Chemistry ; Chondroitin sulfate ; Collagen ; Comparative analysis ; Dermatan sulfate ; Drug dosages ; Engineering Science with specialization in Materials Science ; Extracellular matrix ; Fractures ; Glycosaminoglycans ; Growth factors ; Heparin ; Humans ; Hyaluronic acid ; Hyaluronic Acid - chemistry ; Hyaluronic Acid - pharmacology ; Hydrogels ; Immobilized Proteins - chemistry ; Immobilized Proteins - pharmacology ; Laboratories ; Loading ; Male ; Metabolism ; Nonunion ; Orthopaedics ; Ortopedi ; Ossification (ectopic) ; Osteogenesis ; Osteogenesis - drug effects ; Polymers ; Proteins ; Rats ; Rats, Sprague-Dawley ; Science ; Studies ; Sulfates ; Teknisk fysik med inriktning mot materialvetenskap ; Time Factors ; Tissue engineering
É parte de: PloS one, 2013-10, Vol.8 (10), p.e78551-e78551
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: Jos Buijs, who worked for Science for Life Laboratory, GE Healthcare, Stockholm and currently for Biomedical Radiation Sciences, Uppsala University declares no competing interests. This does not alter the authors' adherence to all the PLOS ONE policies on sharing data and materials.
Conceived and designed the experiments: MK SC JH. Performed the experiments: MK AK MV JB M-KM. Analyzed the data: MK JH JB SC. Contributed reagents/materials/analysis tools: MK JB AK MV JH. Wrote the manuscript: MK JH SC.
Current address: Medical Center, Hospital in Enköping, Enköping, Sweden
Current address: Biomedical Radiation Sciences, Uppsala University, Uppsala, Sweden
Descrição: Bone morphogenetic protein-2 (BMP-2) is considered a promising adjuvant for the treatment of skeletal non-union and spinal fusion. However, BMP-2 delivery in a conventional collagen scaffold necessitates a high dose to achieve an efficacious outcome. To lower its effective dose, we precomplexed BMP-2 with the glycosaminoglycans (GAGs) dermatan sulfate (DS) or heparin (HP), prior to loading it into a hyaluronic acid (HA) hydrogel. In vitro release studies showed that BMP-2 precomplexed with DS or HP had a prolonged delivery compared to without GAG. BMP-2-DS complexes achieved a slightly faster release in the first 24 h than HP; however, both delivered BMP-2 for an equal duration. Analysis of the kinetic interaction between BMP-2 and DS or HP showed that HP had approximately 10 times higher affinity for BMP-2 than DS, yet it equally stabilized the protein, as determined by alkaline phosphatase activity. Ectopic bone formation assays at subcutaneous sites in rats demonstrated that HA hydrogel-delivered BMP-2 precomplexed with GAG induced twice the volume of bone compared with BMP-2 delivered uncomplexed to GAG.
Editor: United States: Public Library of Science
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Complexation and sequestration of BMP-2 from an ECM mimetic hyaluronan gel for improved bone formation

Kisiel, Marta ; Klar, Agnieszka S ; Ventura, Manuela ; Buijs, Jos ; Mafina, Marc-Krystelle ; Cool, Simon M ; Hilborn, Jöns Karamanos, Nikos K

United States: Public Library of Science

Buscando em bases de dados remotas. Favor aguardar.