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Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry

Barreiros, Luisa ; Silva, Eduarda M. P. ; Alencastre, Inês S. ; Lamghari, Meriem ; Segundo, Marcela A.

Analytical and bioanalytical chemistry, 2020-09, Vol.412 (24), p.6625-6632 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

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Título:
Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry
Autor: Barreiros, Luisa ; Silva, Eduarda M. P. ; Alencastre, Inês S. ; Lamghari, Meriem ; Segundo, Marcela A.
Assuntos: Acetonitrile ; Amino acids ; Analytical Chemistry ; Animals ; Arginine - analogs & derivatives ; Arginine - analysis ; Biochemistry ; Bone growth ; Bone marrow ; Breast cancer ; Calibration ; Cell culture ; Characterization and Evaluation of Materials ; Chemistry ; Chemistry and Materials Science ; Chromatography, Liquid - methods ; Culture media ; Data acquisition ; Elution ; Energy balance ; Feeding behavior ; Female Role Models in Analytical Chemistry ; Food Science ; Formic acid ; High performance liquid chromatography ; Homeostasis ; Humans ; Internalization ; Ionization ; Laboratory Medicine ; Limit of Detection ; Liquid chromatography ; Male ; Mass spectrometry ; Mass spectroscopy ; MCF-7 Cells ; Mice, Inbred C57BL ; Monitoring/Environmental Analysis ; Neuropeptide Y ; Neuropeptides ; Nitriles ; Physiological aspects ; Receptors ; Receptors, Neuropeptide Y - analysis ; Receptors, Neuropeptide Y - antagonists & inhibitors ; Regeneration ; Regeneration (physiology) ; Research Paper ; Tandem Mass Spectrometry - methods
É parte de: Analytical and bioanalytical chemistry, 2020-09, Vol.412 (24), p.6625-6632
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
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Descrição: Neuropeptide Y (NPY) is a peptide widely distributed throughout the body that is involved in various physiological processes, including the regulation of feeding behavior and energy homeostasis. 5-Carbamimidamido-2-(2,2-diphenylacetamido)- N -[(4-hydroxyphenyl)methyl]pentanamide (BIBP 3226) is a selective NPY Y1 receptor antagonist with recognized application in bone regeneration studies, requiring quantification at picogram levels. Hence, BIBP 3226 determination is proposed here by a validated HPLC-MS/MS method, based on a reversed-phase Kinetex® core-shell C8 column (2.6 μm, 150 × 2.1 mm) at 30 °C, elution in isocratic mode using a mixture of acetonitrile and water (30:70, v/v), containing 0.1% (v/v) formic acid, at 0.25 mL min −1 , detection in positive ionization mode, and data acquisition in selected reaction monitoring mode. Calibration curves were linear for concentrations ranging from 0.25 to 30 ng mL −1 with LOD and LOQ values as low as 0.1 and 0.3 pg in cell extracts and 16 and 48 pg in supernatant culture media, respectively. BIBP 3226 was successfully determined in cell extracts and supernatants obtained from internalization assays. Using similar exposure conditions, the amount of BIBP 3226 found in breast cancer cells (MCF7) was 72 to 657 times higher than that found in bone marrow cells (Wt C57BL/6 mice), providing an indirect indicator of NPY Y1 receptor expression.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

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Determination of neuropeptide Y Y1 receptor antagonist BIBP 3226 and evaluation of receptor expression based on liquid chromatography coupled with tandem mass spectrometry

Barreiros, Luisa ; Silva, Eduarda M. P. ; Alencastre, Inês S. ; Lamghari, Meriem ; Segundo, Marcela A.

Berlin/Heidelberg: Springer Berlin Heidelberg

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