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Structural and mechanistic insights on nitrate reductases

Coelho, Catarina ; Romão, Maria João

Protein science, 2015-12, Vol.24 (12), p.1901-1911 [Periódico revisado por pares]

United States: Wiley Subscription Services, Inc

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Título:
Structural and mechanistic insights on nitrate reductases
Autor: Coelho, Catarina ; Romão, Maria João
Assuntos: Bacteria ; Bacteria - chemistry ; Bacteria - enzymology ; Bacterial Proteins - chemistry ; Bacterial Proteins - metabolism ; Catalytic Domain ; Cell Membrane - metabolism ; Crystal structure ; Crystallography ; Crystallography, X-Ray ; Cytoplasm - metabolism ; DMSO reductase family ; E coli ; Electrochemistry ; enzymatic mechanism ; Enzymes ; membrane‐bound nitrate reductase ; Metabolism ; Models, Molecular ; molybdenum enzymes ; Nitrate Reductase - chemistry ; Nitrate Reductase - metabolism ; Nitrates ; Nitrogen cycle ; Periplasm - metabolism ; periplasmic nitrate reductase ; Reaction mechanisms ; Reductases ; Reviews ; Structure-function relationships
É parte de: Protein science, 2015-12, Vol.24 (12), p.1901-1911
Notas: Grant number: SFRH/BPD/84581/2012.
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Descrição: Nitrate reductases (NR) belong to the DMSO reductase family of Mo‐containing enzymes and perform key roles in the metabolism of the nitrogen cycle, reducing nitrate to nitrite. Due to variable cell location, structure and function, they have been divided into periplasmic (Nap), cytoplasmic, and membrane‐bound (Nar) nitrate reductases. The first crystal structure obtained for a NR was that of the monomeric NapA from Desulfovibrio desulfuricans in 1999. Since then several new crystal structures were solved providing novel insights that led to the revision of the commonly accepted reaction mechanism for periplasmic nitrate reductases. The two crystal structures available for the NarGHI protein are from the same organism (Escherichia coli) and the combination with electrochemical and spectroscopic studies also lead to the proposal of a reaction mechanism for this group of enzymes. Here we present an overview on the current advances in structural and functional aspects of bacterial nitrate reductases, focusing on the mechanistic implications drawn from the crystallographic data.
Editor: United States: Wiley Subscription Services, Inc
Idioma: Inglês

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Structural and mechanistic insights on nitrate reductases

Coelho, Catarina ; Romão, Maria João

United States: Wiley Subscription Services, Inc

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