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Single‐molecule analysis reveals two distinct states of the compressed RecA filament on single‐stranded DNA

Alekseev, Aleksandr ; Serdakov, Maksim ; Pobegalov, Georgii ; Yakimov, Alexandr ; Bakhlanova, Irina ; Baitin, Dmitry ; Khodorkovskii, Mikhail

FEBS letters, 2020-11, Vol.594 (21), p.3464-3476 [Periódico revisado por pares]

England

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Título:
Single‐molecule analysis reveals two distinct states of the compressed RecA filament on single‐stranded DNA
Autor: Alekseev, Aleksandr ; Serdakov, Maksim ; Pobegalov, Georgii ; Yakimov, Alexandr ; Bakhlanova, Irina ; Baitin, Dmitry ; Khodorkovskii, Mikhail
Assuntos: Adenosine Diphosphate - metabolism ; Adenosine Triphosphate - metabolism ; Apoproteins - chemistry ; Apoproteins - metabolism ; ATP hydrolysis ; DNA, Single-Stranded - chemistry ; DNA, Single-Stranded - metabolism ; DNA-Binding Proteins - chemistry ; DNA-Binding Proteins - metabolism ; Escherichia coli ; Escherichia coli Proteins - chemistry ; Escherichia coli Proteins - metabolism ; homologous recombination ; Rec A Recombinases - chemistry ; Rec A Recombinases - metabolism ; RecA ; Single Molecule Imaging ; single‐molecule biophysics
É parte de: FEBS letters, 2020-11, Vol.594 (21), p.3464-3476
Notas: Edited by Christian Griesinger
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SourceType-Scholarly Journals-1
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Descrição: The RecA protein plays a key role in bacterial homologous recombination (HR) and acts through assembly of long helical filaments around single‐stranded DNA in the presence of ATP. Large‐scale conformational changes induced by ATP hydrolysis result in transitions between stretched and compressed forms of the filament. Here, using a single‐molecule approach, we show that compressed RecA nucleoprotein filaments can exist in two distinct interconvertible states depending on the presence of ADP in the monomer–monomer interface. Binding of ADP promotes cooperative conformational transitions and directly affects mechanical properties of the filament. Our findings reveal that RecA nucleoprotein filaments are able to continuously cycle between three mechanically distinct states that might have important implications for RecA‐mediated processes of HR.
Editor: England
Idioma: Inglês

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Single‐molecule analysis reveals two distinct states of the compressed RecA filament on single‐stranded DNA

Alekseev, Aleksandr ; Serdakov, Maksim ; Pobegalov, Georgii ; Yakimov, Alexandr ; Bakhlanova, Irina ; Baitin, Dmitry ; Khodorkovskii, Mikhail

England

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