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Protein aggregation in E. coli : short term and long term effects of nutrient density

Baig, Ulfat I ; Bhadbhade, Bharati J ; Mariyam, Dincy ; Watve, Milind G Nazir, Aamir

PloS one, 2014-09, Vol.9 (9), p.e107445-e107445 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Protein aggregation in E. coli : short term and long term effects of nutrient density
Autor: Baig, Ulfat I ; Bhadbhade, Bharati J ; Mariyam, Dincy ; Watve, Milind G
Nazir, Aamir
Assuntos: Agglomeration ; Aging ; Antibiotics ; Bacteria ; Biology ; Biology and Life Sciences ; Cell division ; Culture Media ; Diabetes ; Dietary restrictions ; E coli ; Escherichia coli ; Escherichia coli - growth & development ; Escherichia coli - metabolism ; Escherichia coli Proteins - metabolism ; Fluorescence ; Fluorescence microscopy ; Long term effects ; Low calorie ; Metabolic rate ; Nutrients ; Protein Aggregates ; Protein interaction ; Proteins ; Ribonucleic acid ; RNA ; RNA, Bacterial - metabolism ; Science education ; Senescence ; Solubility ; Starvation ; Symmetry
É parte de: PloS one, 2014-09, Vol.9 (9), p.e107445-e107445
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: UB MW. Performed the experiments: UB DM. Analyzed the data: UB DM MW BB. Contributed to the writing of the manuscript: UB MW.
Descrição: During exponential growth some cells of E. coli undergo senescence mediated by asymmetric segregation of damaged components, particularly protein aggregates. We showed previously that functional cell division asymmetry in E. coli was responsive to the nutritional environment. Short term exposure as well as long term selection in low calorie environments led to greater cell division symmetry and decreased frequency of senescent cells as compared to high calorie environments. We show here that long term selection in low nutrient environment decreased protein aggregation as revealed by fluorescence microscopy and proportion of insoluble proteins. Across selection lines protein aggregation was correlated significantly positively with the RNA content, presumably indicating metabolic rate. This suggests that the effects of caloric restriction on cell division symmetry and aging in E. coli may work via altered protein handling mechanisms. The demonstrable effects of long term selection on protein aggregation suggest that protein aggregation is an evolvable phenomenon rather than being a passive inevitable process. The aggregated proteins progressively disappeared on facing starvation indicating degradation and recycling demonstrating that protein aggregation is a reversible process in E. coli.
Editor: United States: Public Library of Science
Idioma: Inglês

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Protein aggregation in E. coli : short term and long term effects of nutrient density

Baig, Ulfat I ; Bhadbhade, Bharati J ; Mariyam, Dincy ; Watve, Milind G Nazir, Aamir

United States: Public Library of Science

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