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Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine

Ma, Lan ; Makino, Yusuke ; Yamaza, Haruyoshi ; Akiyama, Kentaro ; Hoshino, Yoshihiro ; Song, Guangtai ; Kukita, Toshio ; Nonaka, Kazuaki ; Shi, Songtao ; Yamaza, Takayoshi Câmara, Niels Olsen Saraiva

PloS one, 2012-12, Vol.7 (12), p.e51777-e51777 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine
Autor: Ma, Lan ; Makino, Yusuke ; Yamaza, Haruyoshi ; Akiyama, Kentaro ; Hoshino, Yoshihiro ; Song, Guangtai ; Kukita, Toshio ; Nonaka, Kazuaki ; Shi, Songtao ; Yamaza, Takayoshi
Câmara, Niels Olsen Saraiva
Assuntos: Adult ; Age ; Animal tissues ; Animals ; Autoantibodies ; Autoimmune diseases ; Autoimmune Diseases - physiopathology ; Autoimmune Diseases - therapy ; Autoimmunity ; Biocompatibility ; Biology ; Biomarkers - metabolism ; Biomedical materials ; Bone Diseases - pathology ; Bone Diseases - therapy ; Bone growth ; Bone marrow ; Bones ; Cell Proliferation ; Cell self-renewal ; Cellular Microenvironment - immunology ; Child ; Child, Preschool ; Chronic conditions ; Cryopreservation ; Defects ; Dental pulp ; Dental Pulp - cytology ; Dentistry ; Disorders ; Engineering ; Female ; Humans ; Immunological diseases ; Immunology ; Immunomodulation ; Immunotherapy ; Interleukin 17 ; Kidney Function Tests ; Laboratory animals ; Life span ; Long bone ; Lupus ; Lymphocytes ; Lymphocytes T ; Medical research ; Mesenchymal stem cells ; Mice ; Mice, Inbred MRL lpr ; Minerals - metabolism ; Molecular biology ; Multipotent Stem Cells - cytology ; Multipotent Stem Cells - metabolism ; Nephritis ; Osteoporosis ; Pediatrics ; Regeneration - physiology ; Regenerative Medicine ; Renal function ; Science ; Stem Cell Transplantation ; Stem cells ; Stem Cells - cytology ; Stem Cells - metabolism ; Studies ; Systemic lupus erythematosus ; T cells ; Teeth ; Th17 Cells - immunology ; Therapy ; Tissue engineering ; Tooth Exfoliation - pathology ; Tooth, Deciduous - cytology ; Transplantation ; Transplants & implants
É parte de: PloS one, 2012-12, Vol.7 (12), p.e51777-e51777
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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Competing Interests: The authors have declared that no competing interests exist.
Conceived and designed the experiments: TY SS. Performed the experiments: TY LM YM HY KA YH. Analyzed the data: TY LM YM HY KA YH GS KN TK SS. Contributed reagents/materials/analysis tools: TY HY. Wrote the paper: TY.
Descrição: Human exfoliated deciduous teeth have been considered to be a promising source for regenerative therapy because they contain unique postnatal stem cells from human exfoliated deciduous teeth (SHED) with self-renewal capacity, multipotency and immunomodulatory function. However preservation technique of deciduous teeth has not been developed. This study aimed to evaluate that cryopreserved dental pulp tissues of human exfoliated deciduous teeth is a retrievable and practical SHED source for cell-based therapy. SHED isolated from the cryopreserved deciduous pulp tissues for over 2 years (25-30 months) (SHED-Cryo) owned similar stem cell properties including clonogenicity, self-renew, stem cell marker expression, multipotency, in vivo tissue regenerative capacity and in vitro immunomodulatory function to SHED isolated from the fresh tissues (SHED-Fresh). To examine the therapeutic efficacy of SHED-Cryo on immune diseases, SHED-Cryo were intravenously transplanted into systemic lupus erythematosus (SLE) model MRL/lpr mice. Systemic SHED-Cryo-transplantation improved SLE-like disorders including short lifespan, elevated autoantibody levels and nephritis-like renal dysfunction. SHED-Cryo amended increased interleukin 17-secreting helper T cells in MRL/lpr mice systemically and locally. SHED-Cryo-transplantation was also able to recover osteoporosis bone reduction in long bones of MRL/lpr mice. Furthermore, SHED-Cryo-mediated tissue engineering induced bone regeneration in critical calvarial bone-defect sites of immunocompromised mice. The therapeutic efficacy of SHED-Cryo transplantation on immune and skeletal disorders was similar to that of SHED-Fresh. These data suggest that cryopreservation of dental pulp tissues of deciduous teeth provide a suitable and desirable approach for stem cell-based immune therapy and tissue engineering in regenerative medicine.
Editor: United States: Public Library of Science
Idioma: Inglês

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Cryopreserved dental pulp tissues of exfoliated deciduous teeth is a feasible stem cell resource for regenerative medicine

Ma, Lan ; Makino, Yusuke ; Yamaza, Haruyoshi ; Akiyama, Kentaro ; Hoshino, Yoshihiro ; Song, Guangtai ; Kukita, Toshio ; Nonaka, Kazuaki ; Shi, Songtao ; Yamaza, Takayoshi Câmara, Niels Olsen Saraiva

United States: Public Library of Science

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