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Development of a sensitive colorimetric-PCR assay for detection of viruses in woody plants

Rowhani, A. (University of California, Davis.) ; Biardi, L ; Daubert, S.D ; Golino, D.A

Plant disease, 1998-08, Vol.82 (8), p.880-884 [Periódico revisado por pares]

St. Paul, MN: American Phytopathological Society

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Título:
Development of a sensitive colorimetric-PCR assay for detection of viruses in woody plants
Autor: Rowhani, A. (University of California, Davis.) ; Biardi, L ; Daubert, S.D ; Golino, D.A
Assuntos: ANALYTICAL METHODS ; Biological and medical sciences ; CHERRY LEAF ROLL NEPOVIRUS ; Cherry leaf roll virus ; CITRUS SINENSIS ; CITRUS TRISTEZA CLOSTEROVIRUS ; Citrus tristeza virus ; CLOSTEROVIRUS TRISTEZA DEL CITRUS ; CLOSTEROVIRUS TRISTEZA DU CITRUS ; COLORIMETRIA ; COLORIMETRIE ; COLORIMETRY ; CUCUMIS SATIVUS ; DIAGNOSIS ; DIAGNOSTIC ; DIAGNOSTICO ; ENSAYO ; EXPERIMENTACION ; EXPERIMENTATION ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; JUGLANS REGIA ; Microbiology ; NEPOVIRUS ; NEPOVIRUSES ; PCR ; Phytopathology. Animal pests. Plant and forest protection ; PLANT VIRUSES ; Plant viruses and viroids ; POLYMERASE CHAIN REACTION ; Prune dwarf virus ; PRUNUS AVIUM ; Prunus necrotic ringspot virus ; RAPID METHODS ; REVERSE TRANSCRIPTION ; SCREENING ; TECHNIQUE ANALYTIQUE ; Techniques used in virology ; TECNICAS ANALITICAS ; TESTAGE ; TESTING ; TOMATO RINGSPOT NEPOVIRUS ; Tomato ringspot virus ; TRANSCRIPCION INVERSA ; TRANSCRIPTION INVERSE ; Virology ; VIRUS DE LAS PLANTAS ; VIRUS DES VEGETAUX
É parte de: Plant disease, 1998-08, Vol.82 (8), p.880-884
Notas: 1999001478
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Descrição: Diagnostic methods employing the polymerase chain reaction (PCR) provide the most sensitive means currently available for detecting viruses in woody plants. A new technique has been tested that does not rely on gel electrophoresis or molecular hybridization to detect virus-specific PCR products. This colorimetric method for detection of PCR products from woody plants was demonstrated to be at least as sensitive as gel analysis. When combined with immunocapture of virions from plant sap, colorimetric detection provides a means to apply PCR technology to a large number of samples. Here, we report on the use of this technique for detection and quantitation of a walnut isolate of cherry leafroll virus (CLRV-W), citrus tristeza virus (CTV), prune dwarf virus (PDV), prunus necrotic ringspot virus (PNRSV), and tomato ringspot virus (ToRSV) in woody and herbaceous plants. For purified virus preparations, detection limits ranged from 100 pg/ml to 100 ag/ml. We also describe the colorimetric PCR detection of CTV in pooled samples
Editor: St. Paul, MN: American Phytopathological Society
Idioma: Inglês

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Development of a sensitive colorimetric-PCR assay for detection of viruses in woody plants

Rowhani, A. (University of California, Davis.) ; Biardi, L ; Daubert, S.D ; Golino, D.A

St. Paul, MN: American Phytopathological Society

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