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Layer-by-layer ultraviolet assisted extrusion-based (UAE) bioprinting of hydrogel constructs with high aspect ratio for soft tissue engineering applications

Zhuang, Pei ; Ng, Wei Long ; An, Jia ; Chua, Chee Kai ; Tan, Lay Poh Zhao, Feng

PloS one, 2019-06, Vol.14 (6), p.e0216776-e0216776 [Periódico revisado por pares]

United States: Public Library of Science

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Título:
Layer-by-layer ultraviolet assisted extrusion-based (UAE) bioprinting of hydrogel constructs with high aspect ratio for soft tissue engineering applications
Autor: Zhuang, Pei ; Ng, Wei Long ; An, Jia ; Chua, Chee Kai ; Tan, Lay Poh
Zhao, Feng
Assuntos: 3-D printers ; Aerospace engineering ; Animals ; Biocompatibility ; Biology and life sciences ; Biomedical materials ; Bioprinting ; Bioprinting - methods ; Caprolactone ; Cell Line ; Cell Survival ; Cell viability ; Construction materials ; Crosslinking ; Curing ; Engineering ; Engineering and technology ; Extrusion ; Gellan gum ; Graphene ; High aspect ratio ; Hydrogels ; Hydrogels - chemistry ; Innovations ; Integrity ; Mechanical properties ; Medicine and Health Sciences ; Methods ; Mice ; Novels ; Physical Sciences ; Polymer crosslinking ; Polymer industry ; Polymers ; Polysaccharides ; Printing, Three-Dimensional ; Rats ; Rheology ; Soft tissues ; Stem cells ; Technology application ; Three dimensional printing ; Tissue engineering ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Ultraviolet ; Ultraviolet Rays ; Viscosity
É parte de: PloS one, 2019-06, Vol.14 (6), p.e0216776-e0216776
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Descrição: One of the major challenges in the field of soft tissue engineering using bioprinting is fabricating complex tissue constructs with desired structure integrity and mechanical property. To accomplish such requirements, most of the reported works incorporated reinforcement materials such as poly(ϵ-caprolactone) (PCL) polymer within the 3D bioprinted constructs. Although this approach has made some progress in constructing soft tissue-engineered scaffolds, the mechanical compliance mismatch and long degradation period are not ideal for soft tissue engineering. Herein, we present a facile bioprinting strategy that combines the rapid extrusion-based bioprinting technique with an in-built ultraviolet (UV) curing system to facilitate the layer-by-layer UV curing of bioprinted photo-curable GelMA-based hydrogels to achieve soft yet stable cell-laden constructs with high aspect ratio for soft tissue engineering. GelMA is supplemented with a viscosity enhancer (gellan gum) to improve the bio-ink printability and shape fidelity while maintaining the biocompatibility before crosslinking via a layer-by-layer UV curing process. This approach could eventually fabricate soft tissue constructs with high aspect ratio (length to diameter) of ≥ 5. The effects of UV source on printing resolution and cell viability were also studied. As a proof-of-concept, small building units (3D lattice and tubular constructs) with high aspect ratio are fabricated. Furthermore, we have also demonstrated the ability to perform multi-material printing of tissue constructs with high aspect ratio along both the longitudinal and transverse directions for potential applications in tissue engineering of soft tissues. This layer-by-layer ultraviolet assisted extrusion-based (UAE) Bioprinting may provide a novel strategy to develop soft tissue constructs with desirable structure integrity.
Editor: United States: Public Library of Science
Idioma: Inglês

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Layer-by-layer ultraviolet assisted extrusion-based (UAE) bioprinting of hydrogel constructs with high aspect ratio for soft tissue engineering applications

Zhuang, Pei ; Ng, Wei Long ; An, Jia ; Chua, Chee Kai ; Tan, Lay Poh Zhao, Feng

United States: Public Library of Science

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