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WD60/FAP163 is a dynein intermediate chain required for retrograde intraflagellar transport in cilia

Patel-King, Ramila S ; Gilberti, Renée M ; Hom, Erik F Y ; King, Stephen M Holzbaur, Erika

Molecular biology of the cell, 2013-09, Vol.24 (17), p.2668-2677 [Periódico revisado por pares]

United States: The American Society for Cell Biology

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Título:
WD60/FAP163 is a dynein intermediate chain required for retrograde intraflagellar transport in cilia
Autor: Patel-King, Ramila S ; Gilberti, Renée M ; Hom, Erik F Y ; King, Stephen M
Holzbaur, Erika
Assuntos: Animals ; Biological Transport ; Chlamydomonas reinhardtii - genetics ; Chlamydomonas reinhardtii - metabolism ; Cilia - genetics ; Cilia - metabolism ; DNA, Plant ; Dyneins - genetics ; Dyneins - isolation & purification ; Dyneins - metabolism ; Flagella - metabolism ; Molecular Motor Proteins - genetics ; Molecular Motor Proteins - isolation & purification ; Molecular Motor Proteins - metabolism ; Phylogeny ; Planarians - genetics ; Planarians - metabolism ; Plant Proteins - genetics ; Plant Proteins - metabolism ; Protein Transport ; Sequence Alignment
É parte de: Molecular biology of the cell, 2013-09, Vol.24 (17), p.2668-2677
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Retrograde intraflagellar transport (IFT) is required for assembly of cilia. We identify a Chlamydomonas flagellar protein (flagellar-associated protein 163 [FAP163]) as being closely related to the D1bIC(FAP133) intermediate chain (IC) of the dynein that powers this movement. Biochemical analysis revealed that FAP163 is present in the flagellar matrix and is actively trafficked by IFT. Furthermore, FAP163 copurified with D1bIC(FAP133) and the LC8 dynein light chain, indicating that it is an integral component of the retrograde IFT dynein. To assess the functional role of FAP163, we generated an RNA interference knockdown of the orthologous protein (WD60) in planaria. The Smed-wd60(RNAi) animals had a severe ciliary assembly defect that dramatically compromised whole-organism motility. Most cilia were present as short stubs that had accumulated large quantities of IFT particle-like material between the doublet microtubules and the membrane. The few remaining approximately full-length cilia had a chaotic beat with a frequency reduced from 24 to ∼10 Hz. Thus WD60/FAP163 is a dynein IC that is absolutely required for retrograde IFT and ciliary assembly.
Editor: United States: The American Society for Cell Biology
Idioma: Inglês

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WD60/FAP163 is a dynein intermediate chain required for retrograde intraflagellar transport in cilia

Patel-King, Ramila S ; Gilberti, Renée M ; Hom, Erik F Y ; King, Stephen M Holzbaur, Erika

United States: The American Society for Cell Biology

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