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In vitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone

Choudhary, Preeti ; Kataria, Vinod

In vitro cellular & developmental biology. Plant, 2022-06, Vol.58 (3), p.372-381 [Periódico revisado por pares]

Heidelberg: Springer Nature B.V

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  • Título:
    In vitro culture in combination with aeroponics is an efficient means of mass propagation of Sarcostemma acidum: a rare medicinal plant of Indian arid zone
  • Autor: Choudhary, Preeti ; Kataria, Vinod
  • Assuntos: Aeroponics ; Arid zones ; Aridity ; Cuttings ; Desert plants ; Explants ; Gram-positive bacteria ; Greenhouses ; Herbal medicine ; Kinetin ; Medicinal plants ; Micropropagation ; Plantlets ; Propagation ; Rooting ; Roots ; Sarcostemma ; Shoots ; Stems ; Sucrose
  • É parte de: In vitro cellular & developmental biology. Plant, 2022-06, Vol.58 (3), p.372-381
  • Descrição: In vitro culture in combination with aeroponics is observed to be an efficient means for mass propagation of Sarcostemma acidum in the present investigation. S. acidum is a rare leafless xerophytic shrub of the family Apocynaceae, commonly known as Som-lata or khir-kheep, a source of medicines and the religious drink "Somaras." Aeroponic technique was used for the induction of adventitious roots from stem cuttings of field-grown plants for the development of quality plants in the greenhouse as a source of explants for micropropagation. Among various concentrations of IBA, NAA and NOA used for induction of adventitious rooting from stem cuttings 3.0 g L-1 NAA were found to be most suitable with average 90% induction response and 14 adventitious roots. Surface-sterilized nodal shoot segments of 6 to 7 cm length were inoculated on basic and modified Murashige and Skoog's medium containing 3% (w/v) sucrose, 0.8% (w/v) agar, and various concentrations of BAP and kinetin for activation of axillary shoot buds. Shoots were mass multiplied on a modified MS (MMS) medium containing 3.5 mg L-1 BAP and 0.01 mg L-1 IAA. In vitro multiplied shoots were rooted in vitro on half-strength MS medium containing 0.25 mg L-1 NAA. Ninety percent of in vitro regenerated plantlets were hardened successfully on Soilrite in the greenhouse and survived on garden and field soils. This protocol will be useful for in vitro propagation of S. acidum for mass plantations in the desert ecosystem for prospects.
  • Editor: Heidelberg: Springer Nature B.V
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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