Idioma:

Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin

BEN-BARAK, Y ; RUSSELL, J. T ; WHITNALL, M ; OZATO, K ; GAINER, H

Cellular and molecular neurobiology, 1984-12, Vol.4 (4), p.339-349 [Periódico revisado por pares]

New York, NY: Springer

Texto completo disponível

Citações Citado por

Enviar para

Título:
Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin
Autor: BEN-BARAK, Y ; RUSSELL, J. T ; WHITNALL, M ; OZATO, K ; GAINER, H
Assuntos: Analytical, structural and metabolic biochemistry ; Animals ; Antibodies, Monoclonal ; Binding and carrier proteins ; Biological and medical sciences ; Cattle ; Cross Reactions ; Epitopes - analysis ; Fishes ; Fundamental and applied biological sciences. Psychology ; Humans ; Mice - immunology ; monoclonal antibodies ; neurophysins ; Neurophysins - immunology ; Phylogeny ; pituitary (posterior) ; Pituitary Gland, Posterior - immunology ; Proteins ; Rats ; Rats, Inbred Strains ; Species Specificity ; Vertebrata ; Xenopus laevis
É parte de: Cellular and molecular neurobiology, 1984-12, Vol.4 (4), p.339-349
Notas: ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
Descrição: Previously described mouse monoclonal antibodies against rat neurophysins [Ben-Barak, Y., et al. (1985); Whitnall, M. H., et al. (1985)] were studied here for their cross-reactivities to neurophysins (NPs) from other vertebrate species. Posterior pituitary extracts from various mammals (rat, mouse, cow, human) and lower vertebrates (frog, ratfish) were studied. The monoclonal antibodies displayed several distinct patterns of cross-reactivity to the various species, indicating that the epitopes which they recognized were different. PS 67 bound strongly to rat pituitary extract in solid-phase radioimmunoassay (RIA) but showed no cross-reactivity with extracts from any of the other species tested, including the mouse. PS 36 cross-reacted with mouse and frog extracts but showed almost no cross-reactivity with cow and none to ratfish extracts. PS 41 cross-reacted with mouse, cow, and frog extracts. PS 45 was the most cross-reactive antibody and recognized an antigen in extracts from mouse, cow, frog, and ratfish pituitaries. Electrophoresis of proteins extracted from posterior pituitaries, followed by immunoblot staining with either PS 36 or PS 45, demonstrated that the NP-like molecules within each species are heterogeneous, i.e., more than two bands stained in each species. The frog NP stained by PS 45 was about twice the molecular weight of the mammalian NPs. The possible valve of the PS 45 antibody for future molecular cloning experiments on the arginine vasotocin precursor in lower vertebrates is discussed.
Editor: New York, NY: Springer
Idioma: Inglês

Links

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Phylogenetic cross-reactivities of monoclonal antibodies produced against rat neurophysin

BEN-BARAK, Y ; RUSSELL, J. T ; WHITNALL, M ; OZATO, K ; GAINER, H

New York, NY: Springer

Buscando em bases de dados remotas. Favor aguardar.