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Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3′ end

Bernards, André ; Lex, H.T. ; Van der Ploeg, A. ; Frasch, Carlos C. ; Borst, Piet ; Boothroyd, John C. ; Coleman, Sharon ; Cross, George A.M.

Cell, 1981-01, Vol.27 (3), p.497-505 [Periódico revisado por pares]

United States: Elsevier Inc

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  • Título:
    Activation of trypanosome surface glycoprotein genes involves a duplication-transposition leading to an altered 3′ end
  • Autor: Bernards, André ; Lex, H.T. ; Van der Ploeg, A. ; Frasch, Carlos C. ; Borst, Piet ; Boothroyd, John C. ; Coleman, Sharon ; Cross, George A.M.
  • Assuntos: Amino Acid Sequence ; Animals ; Base Sequence ; DNA Transposable Elements ; Gene Expression Regulation ; Genes, Protozoan ; Molecular Sequence Data ; Multigene Family ; Trypanosoma brucei ; Trypanosoma brucei brucei - genetics ; Variant Surface Glycoproteins, Trypanosoma - genetics
  • É parte de: Cell, 1981-01, Vol.27 (3), p.497-505
  • Notas: ObjectType-Article-2
    SourceType-Scholarly Journals-1
    ObjectType-Feature-1
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    ObjectType-Article-1
    ObjectType-Feature-2
  • Descrição: Expression of the genes for variant surface glycoproteins 117 and 118 in Trypanosoma brucei is accompanied by the appearance of an extra copy of these genes, the expression-linked copy, which differs in the surrounding restriction enzyme sites from the corresponding basic copy of the genes. We present direct evidence that the expression-linked copy is the one used for messenger RNA synthesis. By S1-nuclease-protection experiments we show that cloned basic-copy genes contain the nucleotide sequence of the corresponding messenger RNA except for the last 100 to 150 nucleotides before the poly(A) tail. Comparison of the 3′-terminal sequence of the 117 basic-copy gene and the 117 complementary DNA shows that this region differs by multiple point mutations, insertions and deletions, the differences starting within the coding sequence. Genomic blots demonstrate that a Bsp I site in the 3′-terminal part of the 118 complementary DNA is present in the expression-linked copy but not in the basic-copy gene. We conclude that expression-linked copies are the active genes, and that the generation of expression-linked copies involves a duplication-transposition in which the 3′ end of the gene is replaced.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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