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Tumor organoid-T-cell coculture systems

Cattaneo, Chiara M ; Dijkstra, Krijn K ; Fanchi, Lorenzo F ; Kelderman, Sander ; Kaing, Sovann ; van Rooij, Nienke ; van den Brink, Stieneke ; Schumacher, Ton N ; Voest, Emile E

Nature protocols, 2020-01, Vol.15 (1), p.15-39 [Periódico revisado por pares]

England: Nature Publishing Group

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Tumor organoid-T-cell coculture systems
Autor: Cattaneo, Chiara M ; Dijkstra, Krijn K ; Fanchi, Lorenzo F ; Kelderman, Sander ; Kaing, Sovann ; van Rooij, Nienke ; van den Brink, Stieneke ; Schumacher, Ton N ; Voest, Emile E
Assuntos: Antigens ; Blood ; Cancer ; Cancer immunotherapy ; Care and treatment ; CD8 antigen ; Cell culture ; Coculture Techniques - methods ; Colorectal carcinoma ; Degranulation ; Humans ; Immunotherapy ; Lung cancer ; Lymphocytes ; Lymphocytes T ; Methods ; Microsatellites ; Neoplasms - pathology ; Non-small cell lung carcinoma ; Oncology, Experimental ; Organoids ; Organoids - pathology ; Peripheral blood ; Small cell lung carcinoma ; T cells ; T-Lymphocytes - cytology ; Tissue culture ; Tumor cells ; Tumors ; γ-Interferon
É parte de: Nature protocols, 2020-01, Vol.15 (1), p.15-39
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Descrição: T cells are key players in cancer immunotherapy, but strategies to expand tumor-reactive cells and study their interactions with tumor cells at the level of an individual patient are limited. Here we describe the generation and functional assessment of tumor-reactive T cells based on cocultures of tumor organoids and autologous peripheral blood lymphocytes. The procedure consists of an initial coculture of 2 weeks, in which tumor-reactive T cells are first expanded in the presence of (IFNγ-stimulated) autologous tumor cells. Subsequently, T cells are evaluated for their capacity to carry out effector functions (IFNγ secretion and degranulation) after recognition of tumor cells, and their capacity to kill tumor organoids. This strategy is unique in its use of peripheral blood as a source of tumor-reactive T cells in an antigen-agnostic manner. In 2 weeks, tumor-reactive CD8 T-cell populations can be obtained from ~33-50% of samples from patients with non-small-cell lung cancer (NSCLC) and microsatellite-instable colorectal cancer (CRC). This enables the establishment of ex vivo test systems for T-cell-based immunotherapy at the level of the individual patient.
Editor: England: Nature Publishing Group
Idioma: Inglês

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Tumor organoid-T-cell coculture systems

Cattaneo, Chiara M ; Dijkstra, Krijn K ; Fanchi, Lorenzo F ; Kelderman, Sander ; Kaing, Sovann ; van Rooij, Nienke ; van den Brink, Stieneke ; Schumacher, Ton N ; Voest, Emile E

England: Nature Publishing Group

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