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RNA-Seq analysis reveals transcript diversity and active genes after common cutworm (Spodoptera litura Fabricius) attack in resistant and susceptible wild soybean lines

Du, Haiping ; Li, Xiao ; Ning, Lihua ; Qin, Rui ; Du, Qing ; Wang, Qing ; Song, Haina ; Huang, Fang ; Wang, Hui ; Yu, Deyue

BMC genomics, 2019-03, Vol.20 (1), p.237-17, Article 237 [Periódico revisado por pares]

England: BioMed Central Ltd

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RNA-Seq analysis reveals transcript diversity and active genes after common cutworm (Spodoptera litura Fabricius) attack in resistant and susceptible wild soybean lines
Autor: Du, Haiping ; Li, Xiao ; Ning, Lihua ; Qin, Rui ; Du, Qing ; Wang, Qing ; Song, Haina ; Huang, Fang ; Wang, Hui ; Yu, Deyue
Assuntos: Acids ; Animals ; Binding sites ; Breeding ; Cellular signal transduction ; Common cutworm ; Cotton ; Cultivars ; Disease resistance ; Disease susceptibility ; Diseases and pests ; DNA binding proteins ; Encyclopedias ; Environmental aspects ; Gene expression ; Gene Expression Profiling ; Gene Ontology ; Genes ; Genetic aspects ; Genetic diversity ; Genetic engineering ; Genetic Variation ; Genetically engineered foods ; Genetically modified crops ; Genomes ; Genomics ; Glycine ; Glycine max ; Glycine max - genetics ; Glycine max - immunology ; Glycine soja ; Insects ; Jasmonic acid ; Lepidoptera ; Metabolism ; Nutrition ; Pattern analysis ; Phosphate transporter ; Phosphates ; Photosynthesis ; Physiological aspects ; Plant biochemistry ; Plant genetics ; Production management ; Protease inhibitors ; Proteases ; Proteinase inhibitors ; Resistance to insects ; Ribonucleic acid ; RNA ; RNA sequencing ; RNA, Messenger - genetics ; RNA-Seq analysis ; Sequence Analysis, RNA ; Soybean ; Soybeans ; Spatial analysis ; Spodoptera - physiology ; Spodoptera litura ; Transcription (Genetics) ; Transcription factors ; Transcription Factors - genetics ; Transduction ; Transgenic plants ; Wild soybean (Glycine soja Sieb. & Zucc.)
É parte de: BMC genomics, 2019-03, Vol.20 (1), p.237-17, Article 237
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Common cutworm (CCW) is highly responsible for destabilizing soybean productivity. Wild soybean is a resource used by breeders to discover elite defensive genes. The transcriptomes of two wild accessions (W11 and W99) with different resistance to CCW were analyzed at early- and late-induction time points. After induction, the susceptible accession W11 differentially expressed 1268 and 508 genes at the early and late time points, respectively. Compared with W11, the resistant accession W99 differentially expressed 1270 genes at the early time point and many more genes (2308) at the late time point. In total, 3836 non-redundant genes were identified in both lines. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed that the differentially expressed genes (DEGs) in W99 at the late time point were mostly associated with specific processes and pathways. Among the non-redundant genes, 146 genes were commonly up-regulated in the treatment condition compared with the control condition at the early- and late-induction time points in both accessions used in this experiment. Approximately 40% of the common DEGs were related to secondary metabolism, disease resistance, and signal transduction based on their putative function. Excluding the common DEGs, W99 expressed more unique DEGs than W11. Further analysis of the 3836 DEGs revealed that the induction of CCW not only up-regulated defense-related genes, including 37 jasmonic acid (JA)-related genes, 171 plant-pathogen-related genes, and 17 genes encoding protease inhibitors, but also down-regulated growth-related genes, including 35 photosynthesis-related genes, 48 nutrition metabolism genes, and 28 auxin metabolism genes. Therefore, representative defense-related and growth-related genes were chosen for binding site prediction via co-expression of transcription factors (TFs) and spatial expression pattern analyses. In total, 53 binding sites of 28 TFs were identified based on 3 defense-related genes and 3 growth-related genes. Phosphate transporter PT1, which is a representative growth-related gene, was transformed into soybean, and the transgenic soybean plants were susceptible to CCW. In summary, we described transcriptome reprograming after herbivore induction in wild soybean, identified the susceptibility of growth-related genes, and provided new resources for the breeding of herbivore-resistant cultivated soybeans.
Editor: England: BioMed Central Ltd
Idioma: Inglês

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RNA-Seq analysis reveals transcript diversity and active genes after common cutworm (Spodoptera litura Fabricius) attack in resistant and susceptible wild soybean lines

Du, Haiping ; Li, Xiao ; Ning, Lihua ; Qin, Rui ; Du, Qing ; Wang, Qing ; Song, Haina ; Huang, Fang ; Wang, Hui ; Yu, Deyue

England: BioMed Central Ltd

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