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Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles

Manukhova, Tatiana I ; Evtushenko, Ekaterina A ; Ksenofontov, Alexander L ; Arutyunyan, Alexander M ; Kovalenko, Angelina O ; Nikitin, Nikolai A ; Karpova, Olga V Barnwal, Ravi Pratap

PloS one, 2021-07, Vol.16 (7), p.e0255378-e0255378 [Peer Reviewed Journal]

United States: Public Library of Science

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Title:
Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles
Author: Manukhova, Tatiana I ; Evtushenko, Ekaterina A ; Ksenofontov, Alexander L ; Arutyunyan, Alexander M ; Kovalenko, Angelina O ; Nikitin, Nikolai A ; Karpova, Olga V
Barnwal, Ravi Pratap
Subjects: Adsorption ; Alternanthera ; Amino acids ; Biochemical analysis ; Biology ; Biology and Life Sciences ; Circular Dichroism ; Conformation ; Dichroism ; Fluorescence ; Fluorescence spectroscopy ; Labeling ; Medicine and Health Sciences ; Microscopy ; Microscopy, Electron, Transmission ; Nanoparticles ; Nicotiana - virology ; Physical Sciences ; Physiological aspects ; Plant viruses ; Potexvirus - genetics ; Potexvirus - physiology ; Potexviruses ; Proteins ; Research and analysis methods ; RNA, Viral - chemistry ; RNA, Viral - isolation & purification ; RNA, Viral - metabolism ; Spectrometry, Fluorescence ; Spectroscopy ; Temperature ; Virion - chemistry ; Virion - physiology ; Virions ; Virology ; Virus research ; Virus-like particles ; Viruses
Is Part Of: PloS one, 2021-07, Vol.16 (7), p.e0255378-e0255378
Notes: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Competing Interests: The authors have declared that no competing interests exist.
Description: The present work addresses the thermal remodelling of flexible plant viruses with a helical structure and virus-like particles (VLPs). Here, for the first time, the possibility of filamentous Alternanthera mosaic virus (AltMV) virions' thermal transition into structurally modified spherical particles (SP) has been demonstrated. The work has established differences in formation conditions of SP from virions (SPV) and VLPs (SPVLP) that are in accordance with structural data (on AltMV virions and VLPs). SP originate from AltMV virions through an intermediate stage. However, the same intermediate stage was not detected during AltMV VLPs' structural remodelling. According to the biochemical analysis, AltMV SPV consist of protein and do not include RNA. The structural characterisation of AltMV SPV/VLP by circular dichroism, intrinsic fluorescence spectroscopy and thioflavin T fluorescence assay has been performed. AltMV SPV/VLP adsorption properties and the availability of chemically reactive surface amino acids have been analysed. The revealed characteristics of AltMV SPV/VLP indicate that they could be applied as protein platforms for target molecules presentation and for the design of functionally active complexes.
Publisher: United States: Public Library of Science
Language: English

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Thermal remodelling of Alternanthera mosaic virus virions and virus-like particles into protein spherical particles

Manukhova, Tatiana I ; Evtushenko, Ekaterina A ; Ksenofontov, Alexander L ; Arutyunyan, Alexander M ; Kovalenko, Angelina O ; Nikitin, Nikolai A ; Karpova, Olga V Barnwal, Ravi Pratap

United States: Public Library of Science

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