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Effects of photoreactivation of cyclobutane pyrimidine dimers and pyrimidine (6–4) pyrimidone photoproducts on ultraviolet mutagenesis in SOS-induced repair-deficient Escherichia coli

Tanaka, Masashi ; Nakajima, Satoshi ; Ihara, Makoto ; Matsunaga, Tsukasa ; Nikaido, Osamu ; Yamamoto, Kazuo

Mutagenesis, 2001-01, Vol.16 (1), p.1-6 [Periódico revisado por pares]

Oxford: Oxford University Press

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Título:
Effects of photoreactivation of cyclobutane pyrimidine dimers and pyrimidine (6–4) pyrimidone photoproducts on ultraviolet mutagenesis in SOS-induced repair-deficient Escherichia coli
Autor: Tanaka, Masashi ; Nakajima, Satoshi ; Ihara, Makoto ; Matsunaga, Tsukasa ; Nikaido, Osamu ; Yamamoto, Kazuo
Assuntos: Base Sequence ; Biological and medical sciences ; cyclobutane pyrimidine dimers ; Deoxyribodipyrimidine Photo-Lyase - metabolism ; DNA Repair - genetics ; DNA Repair - radiation effects ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Bacterial - radiation effects ; Escherichia coli ; Escherichia coli - enzymology ; Escherichia coli - genetics ; Escherichia coli - radiation effects ; Fundamental and applied biological sciences. Psychology ; Genes, Suppressor ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mutagenesis ; Mutagenesis. Repair ; Photochemistry ; photolyase ; photoproducts ; Plasmids - genetics ; Plasmids - metabolism ; Plasmids - radiation effects ; pyrimidine (6-4) pyrimidone ; Pyrimidine Dimers - chemistry ; Pyrimidine Dimers - genetics ; Pyrimidine Dimers - radiation effects ; RNA, Transfer - genetics ; RNA, Transfer - metabolism ; RNA, Transfer - radiation effects ; SOS Response (Genetics) - genetics ; SOS Response (Genetics) - radiation effects ; supF gene ; transversion ; Ultraviolet Rays
É parte de: Mutagenesis, 2001-01, Vol.16 (1), p.1-6
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Descrição: Using purified photolyases for pyrimidine (6–4) pyrimidone photoproducts [(6–4)PP] and cyclobutane pyrimidine dimers (CPD), the effects of photoreactivation on mutagenesis were examined in the supF gene on a plasmid transfected into repair-deficient SOS-induced Escherichia coli host cells. More than 95% of CPD and (6–4)PP were removed from plasmid DNA by treatment with CPD photolyase and (6–4)photolyase, respectively. In each photolyase treatment, base substitutions at dipyrimidine sequences were predominantly observed. Of the singlebase substitutions observed after CPD photoreactivation, 83% were A:T→G:C transitions at 5′-TT-3′ sites. After (6–4)photolyase treatment, 81% were G:C→A:T transitions at 5′-CC-3′ and 5′-TC-3′ sequences. Thus, the major mutagenic photoproducts of single-base substitutions were CPD at 5′-CC-3′ or 5′-TC-3′ sites and (6–4)PP at 5′-TT-3′ sites. Tandem double mutations occurred mainly at 5′-CC-3′ sites and were CPD-photoreactivated, suggesting that CPD at 5′-CC-3′ was responsible for tandem double mutations. After photoreactivation of both CPD and (6–4)PP, single-base substitutions were primarily G:C→A:T transitions at 5′-CC-3′ or 5′-TC-3′ sites and A:T→G:C transitions at 5′-TT-3′ sites, and secondarily G:C→T:A transversions at 5′-CC-3′ sites, G:C→C:G transversions at 5′-CC-3′ sites and A:T→T:A transversions at 5′-TT-3′ sites, which were essentially the same as those observed after photoreactivation of CPD alone, (6–4)PP alone and without photoreactivation. Thus, these transversions were not derived from unknown UV adducts but from incompletely repaired CPD and (6–4)PP.
Editor: Oxford: Oxford University Press
Idioma: Inglês

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Effects of photoreactivation of cyclobutane pyrimidine dimers and pyrimidine (6–4) pyrimidone photoproducts on ultraviolet mutagenesis in SOS-induced repair-deficient Escherichia coli

Tanaka, Masashi ; Nakajima, Satoshi ; Ihara, Makoto ; Matsunaga, Tsukasa ; Nikaido, Osamu ; Yamamoto, Kazuo

Oxford: Oxford University Press

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