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Antimicrobial efficacy of in vitro and ex vivo photodynamic therapy using porphyrins against Moraxella spp. isolated from bovine keratoconjunctivitis

Seeger, M. G. ; Machado, C. S. ; Iglesias, B. A. ; Vogel, F. S. F. ; Cargnelutti, J. F.

World journal of microbiology & biotechnology, 2022-06, Vol.38 (6), p.103-103, Article 103 [Periódico revisado por pares]

Dordrecht: Springer Netherlands

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Antimicrobial efficacy of in vitro and ex vivo photodynamic therapy using porphyrins against Moraxella spp. isolated from bovine keratoconjunctivitis
Autor: Seeger, M. G. ; Machado, C. S. ; Iglesias, B. A. ; Vogel, F. S. F. ; Cargnelutti, J. F.
Assuntos: Animals ; Anti-Bacterial Agents - pharmacology ; Anti-Infective Agents ; Antibacterial activity ; Applied Microbiology ; Biochemistry ; Biomedical and Life Sciences ; Biotechnology ; Cattle ; Cattle Diseases - microbiology ; Clinical isolates ; Cornea ; Deactivation ; Economic impact ; Environmental Engineering/Biotechnology ; Etiology ; Eye diseases ; In vivo methods and tests ; Inactivation ; Irradiation ; Keratoconjunctivitis ; Keratoconjunctivitis, Infectious - drug therapy ; Keratoconjunctivitis, Infectious - microbiology ; Life Sciences ; Microbiology ; Microorganisms ; Moraxella ; Moraxellaceae Infections - drug therapy ; Moraxellaceae Infections - microbiology ; Moraxellaceae Infections - veterinary ; Original Paper ; Photochemotherapy ; Photodynamic therapy ; Photosensitization ; Porphyrins ; Porphyrins - pharmacology ; Radiation ; Sheep ; Strains (organisms)
É parte de: World journal of microbiology & biotechnology, 2022-06, Vol.38 (6), p.103-103, Article 103
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SourceType-Scholarly Journals-1
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Descrição: Infectious bovine keratoconjunctivitis (IBK) is an ocular disease affecting bovine herds worldwide, and it causes significant economic loss. The etiologic agent of IBK is considered to be Moraxella bovis , but M. ovis and M. bovoculi are frequently recovered of animals presenting clinical signs of IBK. The therapeutic measures available for its control have limited efficacy. Antimicrobial photodynamic therapy (aPDT) using porphyrins as photosensitizing molecules is an alternative method that can be used to reduce microbial growth. We evaluated the antibacterial activity of aPDT using two water-soluble tetra-cationic porphyrins ( H 2 TMeP and ZnTMeP ) against 22 clinical isolates and standard strains of Moraxella spp. in vitro and in an ex vivo model. For the in vitro assay, 4.0 µM of porphyrin was incubated with approximately 1.0 × 10 4 CFU/mL of each Moraxella sp. isolate and exposed to artificial light for 0, 2.5, 5, and 7.5 min. Next, 50 µL of this solution was plated and incubated for 24 h until CFU measurement. For the ex vivo assay, corneas excised from the eyeballs of slaughtered cattle were irrigated with Moraxella spp. culture, followed by the addition of zinc(II) porphyrin ZnTMeP (4.0 μM). The corneal samples were irradiated for 0, 7.5, and 30 min, followed by swab collection, plating, and CFU count. The results demonstrated the in vitro inactivation of the strains and clinical isolates of Moraxella spp. after 2.5 min of irradiation using ZnTMeP , reaching complete inactivation until 7.5 min. In the ex vivo experiment, the use of ZnTMeP resulted in the most significant reduction in bacterial concentration after 30 min of irradiation. These results encourage future in vivo experiments to investigate the role of metalloporphyrin ZnTMeP in the inactivation of Moraxella spp. isolates causing IBK.
Editor: Dordrecht: Springer Netherlands
Idioma: Inglês

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Antimicrobial efficacy of in vitro and ex vivo photodynamic therapy using porphyrins against Moraxella spp. isolated from bovine keratoconjunctivitis

Seeger, M. G. ; Machado, C. S. ; Iglesias, B. A. ; Vogel, F. S. F. ; Cargnelutti, J. F.

Dordrecht: Springer Netherlands

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