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Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection

Bencke-Malato, Marta ; Cabreira, Caroline ; Wiebke-Strohm, Beatriz ; Bücker-Neto, Lauro ; Mancini, Estefania ; Osorio, Marina B ; Homrich, Milena S ; Turchetto-Zolet, Andreia Carina ; De Carvalho, Mayra CCG ; Stolf, Renata ; Weber, Ricardo LM ; Westergaard, Gastón ; Castagnaro, Atílio P ; Abdelnoor, Ricardo V ; Marcelino-GuimarÃ£es, Francismar C ; Margis-Pinheiro, Márcia ; Bodanese-Zanettini, Maria Helena

BMC plant biology, 2014-09, Vol.14 (1), Article 236 [Periódico revisado por pares]

BioMed Central Ltd

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Título:
Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection
Autor: Bencke-Malato, Marta ; Cabreira, Caroline ; Wiebke-Strohm, Beatriz ; Bücker-Neto, Lauro ; Mancini, Estefania ; Osorio, Marina B ; Homrich, Milena S ; Turchetto-Zolet, Andreia Carina ; De Carvalho, Mayra CCG ; Stolf, Renata ; Weber, Ricardo LM ; Westergaard, Gastón ; Castagnaro, Atílio P ; Abdelnoor, Ricardo V ; Marcelino-GuimarÃ£es, Francismar C ; Margis-Pinheiro, Márcia ; Bodanese-Zanettini, Maria Helena
Assuntos: Database searching ; DNA binding proteins ; Genetic aspects ; Genetic transcription ; Internet/Web search services ; Medical equipment and supplies industry ; Medical test kit industry ; Online searching ; RNA sequencing
É parte de: BMC plant biology, 2014-09, Vol.14 (1), Article 236
Descrição: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified. As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants. The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants.
Editor: BioMed Central Ltd
Idioma: Inglês

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Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhiziinfection

BioMed Central Ltd

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