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Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

McGlade, Emily A. ; Herrera, Gerardo G. ; Stephens, Kalli K. ; Olsen, Sierra L. W. ; Winuthayanon, Sarayut ; Guner, Joie ; Hewitt, Sylvia C. ; Korach, Kenneth S. ; DeMayo, Francesco J. ; Lydon, John P. ; Monsivais, Diana ; Winuthayanon, Wipawee

The FASEB journal, 2021-05, Vol.35 (5), p.e21563-n/a [Revista revisada por pares]

United States

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Título:
Cell‐type specific analysis of physiological action of estrogen in mouse oviducts
Autor: McGlade, Emily A. ; Herrera, Gerardo G. ; Stephens, Kalli K. ; Olsen, Sierra L. W. ; Winuthayanon, Sarayut ; Guner, Joie ; Hewitt, Sylvia C. ; Korach, Kenneth S. ; DeMayo, Francesco J. ; Lydon, John P. ; Monsivais, Diana ; Winuthayanon, Wipawee
Materias: Animals ; Biomarkers - metabolism ; embryo development ; embryo transport ; Estradiol - pharmacology ; estrogen ; Estrogens - pharmacology ; Fallopian Tubes - cytology ; Fallopian Tubes - drug effects ; Fallopian Tubes - physiology ; Female ; Gene Expression Profiling ; Gene Expression Regulation, Developmental - drug effects ; Insulin-Like Growth Factor I - physiology ; insulin‐like growth factor 1 ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; oviduct ; Oviducts - cytology ; Oviducts - drug effects ; Oviducts - physiology ; Receptors, Progesterone - physiology ; scRNA‐seq ; Single-Cell Analysis - methods
Es parte de: The FASEB journal, 2021-05, Vol.35 (5), p.e21563-n/a
Notas: E. McGlade, G. Herrera, and W. Winuthayanon designed research; E. McGlade, G. Herrera, K. Stephens, and S. Olsen analyzed data; E. McGlade, G. Herrera, K. Stephens, S. Olsen, J. Guner, S. Hewitt, D. Monsivais, and W. Winuthayanon performed research; E. McGlade, G. Herrera, and W. Winuthayanon wrote the paper; F. DeMayo, J. Lydon, and K. Korach contributed new reagents or analytic tools; and G. Herrera and S. Winuthayanon developed software necessary to perform and record experiments.
AUTHOR CONTRIBUTIONS
Present address Gerardo G. Herrera, Department of Molecular & Integrative Physiology, University of Kansas, 11 Medical Center, Kansas City, KS, USA
Descripción: One of the endogenous estrogens, 17β‐estradiol (E2) is a female steroid hormone secreted from the ovary. It is well established that E2 causes biochemical and histological changes in the uterus. However, it is not completely understood how E2 regulates the oviductal environment in vivo. In this study, we assessed the effect of E2 on each oviductal cell type, using an ovariectomized‐hormone‐replacement mouse model, single‐cell RNA‐sequencing (scRNA‐seq), in situ hybridization, and cell‐type‐specific deletion in mice. We found that each cell type in the oviduct responded to E2 distinctively, especially ciliated and secretory epithelial cells. The treatment of exogenous E2 did not drastically alter the transcriptomic profile from that of endogenous E2 produced during estrus. Moreover, we have identified and validated genes of interest in our datasets that may be used as cell‐ and region‐specific markers in the oviduct. Insulin‐like growth factor 1 (Igf1) was characterized as an E2‐target gene in the mouse oviduct and was also expressed in human fallopian tubes. Deletion of Igf1 in progesterone receptor (Pgr)‐expressing cells resulted in female subfertility, partially due to an embryo developmental defect and embryo retention within the oviduct. In summary, we have shown that oviductal cell types, including epithelial, stromal, and muscle cells, are differentially regulated by E2 and support gene expression changes, such as growth factors that are required for normal embryo development and transport in mouse models. Furthermore, we have identified cell‐specific and region‐specific gene markers for targeted studies and functional analysis in vivo.
Editor: United States
Idioma: Inglés

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Cell‐type specific analysis of physiological action of estrogen in mouse oviducts

McGlade, Emily A. ; Herrera, Gerardo G. ; Stephens, Kalli K. ; Olsen, Sierra L. W. ; Winuthayanon, Sarayut ; Guner, Joie ; Hewitt, Sylvia C. ; Korach, Kenneth S. ; DeMayo, Francesco J. ; Lydon, John P. ; Monsivais, Diana ; Winuthayanon, Wipawee

United States

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