Idioma:

Amplicon-based profiling of bacteria in raw and secondary treated wastewater from treatment plants across Australia

Ahmed, Warish ; Staley, Christopher ; Sidhu, Jatinder ; Sadowsky, Michael ; Toze, Simon

Applied microbiology and biotechnology, 2017-02, Vol.101 (3), p.1253-1266 [Periódico revisado por pares]

Berlin/Heidelberg: Springer Berlin Heidelberg

Texto completo disponível

Citações Citado por

Enviar para

Título:
Amplicon-based profiling of bacteria in raw and secondary treated wastewater from treatment plants across Australia
Autor: Ahmed, Warish ; Staley, Christopher ; Sidhu, Jatinder ; Sadowsky, Michael ; Toze, Simon
Assuntos: Analysis ; Antibiotics ; Applied Microbial and Cell Physiology ; Arcobacter ; Arcobacter - genetics ; Arcobacter - isolation & purification ; Australia ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteria - pathogenicity ; Bacteroides ; Bacteroides - genetics ; Bacteroides - isolation & purification ; Biomedical and Life Sciences ; Biotechnology ; Drug resistance ; Drug Resistance, Microbial - genetics ; E coli ; Health risk assessment ; Health risks ; High-Throughput Nucleotide Sequencing ; Humans ; Life Sciences ; Microbial Consortia - genetics ; Microbial drug resistance ; Microbial Genetics and Genomics ; Microbiology ; Pathogens ; Pseudomonas ; Pseudomonas - genetics ; Pseudomonas - isolation & purification ; Public health ; Ribonucleic acid ; Ribosomal RNA ; Risk Factors ; RNA ; RNA, Ribosomal, 16S ; Sequence Analysis, DNA ; Sewage treatment ; Sludge ; Studies ; Waste Disposal, Fluid ; Waste Water - microbiology ; Water Purification ; Water treatment ; Water treatment plants
É parte de: Applied microbiology and biotechnology, 2017-02, Vol.101 (3), p.1253-1266
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: In this study, we investigated the use of Illumina high-throughput sequencing of 16S ribosomal RNA (rRNA) amplicons to explore microbial diversity and community structure in raw and secondary treated wastewater (WW) samples from four municipal wastewater treatment plants (WWTPs A–D) across Australia. Sequence reads were analyzed to determine the abundance and diversity of bacterial communities in raw and secondary treated WW samples across the four WWTPs. In addition, sequence reads were also characterized to phenotypic features and to estimate the abundance of potential pathogenic bacterial genera and antibiotic-resistant genes in total bacterial communities. The mean coverage, Shannon diversity index, observed richness ( S obs ), and abundance-based coverage estimate (ACE) of richness for raw and secondary treated WW samples did not differ significantly ( P > 0.05) among the four WWTPs examined. Generally, raw and secondary treated WW samples were dominated by members of the genera Pseudomonas , Arcobacter , and Bacteroides . Evaluation of source contributions to secondary treated WW, done using SourceTracker, revealed that 8.80–61.4% of the bacterial communities in secondary treated WW samples were attributed to raw WW. Twenty-five bacterial genera were classified as containing potential bacterial pathogens. The abundance of potentially pathogenic genera in raw WW samples was higher than that found in secondary treated WW samples. Among the pathogenic genera identified, Pseudomonas and Arcobacter had the greatest percentage of the sequence reads. The abundances of antibiotic resistance genes were generally low (<0.5%), except for genes encoding ABC transporters, which accounted for approximately 3% of inferred genes. These findings provided a comprehensive profile of bacterial communities, including potential bacterial pathogens and antibiotic-resistant genes, in raw and secondary treated WW samples from four WWTPs across Australia and demonstrated that Illumina high-throughput sequencing can be an alternative approach for monitoring WW quality in order to protect environmental and human health.
Editor: Berlin/Heidelberg: Springer Berlin Heidelberg
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Resultado 1 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Amplicon-based profiling of bacteria in raw and secondary treated wastewater from treatment plants across Australia

Ahmed, Warish ; Staley, Christopher ; Sidhu, Jatinder ; Sadowsky, Michael ; Toze, Simon

Berlin/Heidelberg: Springer Berlin Heidelberg

Buscando em bases de dados remotas. Favor aguardar.