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Characterization of NOD-like genes in Leishmania

F. A. L. Franco A. F Crispim; Silvia Reni Bortolin Uliana; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)

Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005

São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP 2005

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  • Título:
    Characterization of NOD-like genes in Leishmania
  • Autor: F. A. L. Franco
  • A. F Crispim; Silvia Reni Bortolin Uliana; Congresso do Instituto de Ciências Biomédicas (4. 2005 São Paulo)
  • Assuntos: PARASITOLOGIA
  • É parte de: Resumos São Paulo: Comissão de Cultura e Extensão Universitária do ICB/USP, 2005
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Many of the proteins in the NOD family have been implicated in the intracellular recognition of pathogens. The function of NOD proteins is dependent on the binding and cleavage of nucleosides through domains known as Walker A and B (Nuñez et al., Annu. Rev. Biochem. 74:355-83, 2005). In the process of characterizing the genomic region of the L. amazonensis meta 1 gene (FEMS Microbiol Lett, 238(1):213-9, 2004), we identified an ORF of 2,03 Kb that presented similarity with the human NOD3 protein. Objective: Characterization of NODlike genes in Leishmania. Methods and results: Northern blots indicated that this gene is upregulated in amastigotes of L. amazonensis. The complete nucleotide sequence of the L. amazonensis ORF was obtained. The predicted translated sequence of 675 amino acids was used to identify orthologous L. major and L. infantum proteins (www.genedb.org). The alignment of these sequences revealed high degrees of similarity. The 73-kDa recombinant NOD-like protein was expressed and used to raise specific antibodies that recognized a 45 kDa protein in total extracts of L. amazonensis amastigotes. The search for orthologues on T. brucei and T. cruzi translated genome databanks identified predicted proteins of 478 and 470 amino acids, respectively, with high degrees of similarity with the L. amazonensis protein. The sequence alignment of the 3 peptides showed that T. brucei and T. cruzi proteins lack the C-terminal region
    of the NOD-like protein found in the Leishmania species. A sequence search in the unfinished L. major Friedlin genome project data allowed the identification of 5 further possible NOD-related genes. One of these genes (LmjF 11.0890) encodes a protein containing Walker's A and B domains. The characterization of this gene and of the encoded protein is under way. Conclusion: The characterisation of NOD-like genes in Leishmania may produce the necessary tools to investigate the participation of these proteins in the macrophage-parasite interaction.
  • Editor: São Paulo Comissão de Cultura e Extensão Universitária do ICB/USP
  • Data de criação/publicação: 2005
  • Formato: res. 274.
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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