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Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes

T. R. Campoio T. S Batista; S. B Braga; S Barbosa; J Pires; Rui Curi; R Otton; Reunião Anual da Federação de Sociedades de Biologia Experimental - FESBE (21. 2006 Águas de Lindóia, SP); Congresso Brasileiro de Biofísica (31. 2006 Águas de Lindóia, SP); Congresso Brasileiro de Investigação Clínica (22. 2006 Águas de Lindóia, SP); Congresso Brasileiro de Neurociências e Comportamento (30. 2006 Águas de Lindóia, SP)

Programa e resumos São Paulo: FeSBE, 2006

Águas de Lindóia Federação de Sociedades de Biologia Experimental 2006

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  • Título:
    Involvement of cytochrome C and SMAC/DIABLO release from mitochondria and caspase -3 and -6 activation in fatty acid- induced apoptosis in human blood lymphocytes
  • Autor: T. R. Campoio
  • T. S Batista; S. B Braga; S Barbosa; J Pires; Rui Curi; R Otton; Reunião Anual da Federação de Sociedades de Biologia Experimental - FESBE (21. 2006 Águas de Lindóia, SP); Congresso Brasileiro de Biofísica (31. 2006 Águas de Lindóia, SP); Congresso Brasileiro de Investigação Clínica (22. 2006 Águas de Lindóia, SP); Congresso Brasileiro de Neurociências e Comportamento (30. 2006 Águas de Lindóia, SP)
  • Assuntos: FISIOLOGIA
  • É parte de: Programa e resumos São Paulo: FeSBE, 2006
  • Notas Locais: Disponível somente em CD-ROM
  • Descrição: Treatment with a mixture of fatty acids in similar proportion to that found in human plasma triggers apoptosis in peripheral blood lymphocytes from healthy subjects. The present study was undertaken to evaluate if the effect of a mixture of fatty acids upon normal human lymphocytes involves cytochrome c and Smac/Diablo release from the mitochondria, activation of caspases-3, -6 and -8 and expression of the anti-apoptotic blc-2 and 14 3 3 and the pro-apoptotic bax and FasL proteins. Peripheral blood lymphocytes from healthy subjects were isolated and treated for 24 h with increasing concentrations (0.2 and 0.4 mM) of the fatty acid mixture. Afterwards, cells were harvested and the cytochrome c and Smac/Diablo release from mitochondrial intermembrane space into cytosol and expression of blc-2, 14 3 3, FasL and bax were investigated by Western blotting analysis. Activities of caspases-3, -6 and -8 were determined by using spectrofluorometric assays. Lymphocytes treated with the fatty acid mixture presented increased cytochrome c and Smac/Diablo release from mitochondria as compared with control lymphocytes without treatment. Caspase -3 and -6 activities were increased by 146% and 22%, respectively, by the treatment with 0.4 mM concentration of the fatty acid mixture for 24 h. The expression of bcl-2 protein was significantly reduced in lymphocytes treated with the fatty acid mixture, by 40% at 0.2 mM and by 80% at 0.4 mM fatty acid concentration as well as 14 3 3 protein
    that was decreased by 66% at 0.2 mM and by 23% at 0.4 mM. However, bax and FasL proteins were not affected by the fatty acid treatment. In conclusion, fatty acids induce apoptosis in human lymphocytes through cytochrome c and Smac/Diablo release from the mitochondria, activation of the caspase cascade and by decrease of anti-apoptotic bcl-2 and 14 3 3 proteins content.
  • Editor: Águas de Lindóia Federação de Sociedades de Biologia Experimental
  • Data de criação/publicação: 2006
  • Formato: res. 23.021.
  • Idioma: Português
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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