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Neopterin induces pro‐atherothrombotic phenotype in human coronary endothelial cells

CIRILLO, P. ; PACILEO, M. ; DE ROSA, S. ; CALABRÒ, P. ; GARGIULO, A. ; ANGRI, V. ; GRANATO‐CORIGLIANO, F. ; FIORENTINO, I. ; PREVETE, N. ; DE PALMA, R. ; MAURO, C. ; LEONARDI, A. ; CHIARIELLO, M.

Journal of thrombosis and haemostasis, 2006-10, Vol.4 (10), p.2248-2255 [Periódico revisado por pares]

Oxford, UK: Blackwell Publishing Ltd

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Título:
Neopterin induces pro‐atherothrombotic phenotype in human coronary endothelial cells
Autor: CIRILLO, P. ; PACILEO, M. ; DE ROSA, S. ; CALABRÒ, P. ; GARGIULO, A. ; ANGRI, V. ; GRANATO‐CORIGLIANO, F. ; FIORENTINO, I. ; PREVETE, N. ; DE PALMA, R. ; MAURO, C. ; LEONARDI, A. ; CHIARIELLO, M.
Assuntos: adhesion molecules ; atherosclerosis ; Cell Adhesion ; Coronary Vessels - cytology ; Coronary Vessels - pathology ; Dose-Response Relationship, Drug ; Endothelial Cells - cytology ; Endothelium, Vascular - pathology ; Humans ; Inflammation ; Intercellular Signaling Peptides and Proteins - metabolism ; Neopterin - pharmacology ; NF-kappa B - metabolism ; Phenotype ; RNA, Messenger - metabolism ; thrombosis ; Thrombosis - pathology ; tissue factor ; Transcription, Genetic
É parte de: Journal of thrombosis and haemostasis, 2006-10, Vol.4 (10), p.2248-2255
Descrição: Background: Inflammation plays a pivotal role in atherothrombosis. Recent data indicate that serum levels of neopterin, a marker of inflammation and immune modulator secreted by monocytes/macrophages, are elevated in patients with acute coronary syndromes and seem to be a prognostic marker for major cardiovascular events. The aim of the present study was to determine whether neopterin might affect the thrombotic and atherosclerotic characteristics of human coronary artery endothelial cells (HCAECs). Methods and results: In HCAECs, neopterin induced TF‐mRNA transcription as demonstrated by real time polymerase chain reaction and expression of functionally active tissue factor (TF) as demonstrated by procoagulant activity assay, and of cellular adhesion molecules (CAMs) as demonstrated by FACS analysis, in a dose‐dependent fashion. These neopterin effects were prevented by lovastatin, a HMG‐CoA reductase inhibitor. Neopterin‐induced TF and CAMs expression was mediated by oxygen free radicals through the activation of the transcription factor, nuclear factor‐kappa B (NF‐κB), as demonstrated by electrophoretic mobility shift assay and by suppression of CAMs and TF expression by superoxide dismutase and by NF‐κB inhibitor, pyrrolidine‐dithio‐carbamate ammonium. Conclusions: These data indicate that neopterin exerts direct effects on HCAECs by promoting CAMs and TF expression and support the hypothesis that neopterin, besides representing a marker of inflammation, might be an effector molecule able to induce a pro‐atherothrombotic phenotype in cells of the coronary circulation.
Editor: Oxford, UK: Blackwell Publishing Ltd
Idioma: Inglês

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Neopterin induces pro‐atherothrombotic phenotype in human coronary endothelial cells

CIRILLO, P. ; PACILEO, M. ; DE ROSA, S. ; CALABRÒ, P. ; GARGIULO, A. ; ANGRI, V. ; GRANATO‐CORIGLIANO, F. ; FIORENTINO, I. ; PREVETE, N. ; DE PALMA, R. ; MAURO, C. ; LEONARDI, A. ; CHIARIELLO, M.

Oxford, UK: Blackwell Publishing Ltd

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