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Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR

Sales, Mariana Lázaro ; Fonseca Júnior, Antônio Augusto ; Pinheiro de Oliveira, Tatiana Flávia ; de Araújo Lopes, Amanda ; Rivetti Júnior, Anselmo Vasconcelos ; Camargos, Marcelo Fernandes ; dos Reis, Jenner Karlisson Pimenta

Brazilian journal of microbiology, 2023-03, Vol.54 (1), p.491-497 [Revista revisada por pares]

Cham: Springer International Publishing

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Título:
Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR
Autor: Sales, Mariana Lázaro ; Fonseca Júnior, Antônio Augusto ; Pinheiro de Oliveira, Tatiana Flávia ; de Araújo Lopes, Amanda ; Rivetti Júnior, Anselmo Vasconcelos ; Camargos, Marcelo Fernandes ; dos Reis, Jenner Karlisson Pimenta
Materias: Animals ; Biomedical and Life Sciences ; Cell culture ; Cell lines ; Droplets ; Food Microbiology ; Genotypes ; Life Sciences ; Medical Microbiology ; Microbial Ecology ; Microbial Genetics and Genomics ; Microbiology ; Mycology ; Phenotypes ; Phylogeny ; Polymerase chain reaction ; Replication ; Reverse Transcriptase Polymerase Chain Reaction ; Ribonucleic acid ; RNA ; RNA, Viral - genetics ; Stomatitis ; Vesicular Stomatitis ; Vesiculovirus - genetics ; Veterinary Microbiology - Research Paper ; Viruses
Es parte de: Brazilian journal of microbiology, 2023-03, Vol.54 (1), p.491-497
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Responsible Editor: Luiz Henrique Rosa
Descripción: Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study.
Editor: Cham: Springer International Publishing
Idioma: Inglés

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Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR

Sales, Mariana Lázaro ; Fonseca Júnior, Antônio Augusto ; Pinheiro de Oliveira, Tatiana Flávia ; de Araújo Lopes, Amanda ; Rivetti Júnior, Anselmo Vasconcelos ; Camargos, Marcelo Fernandes ; dos Reis, Jenner Karlisson Pimenta

Cham: Springer International Publishing

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