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ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions

Ambroggio, Ernesto ; Sorre, Benoît ; Bassereau, Patricia ; Goud, Bruno ; Manneville, Jean-Baptiste ; Antonny, Bruno

The EMBO journal, 2010-01, Vol.29 (2), p.292-303 [Periódico revisado por pares]

Chichester, UK: John Wiley & Sons, Ltd

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Título:
ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions
Autor: Ambroggio, Ernesto ; Sorre, Benoît ; Bassereau, Patricia ; Goud, Bruno ; Manneville, Jean-Baptiste ; Antonny, Bruno
Assuntos: ADP-Ribosylation Factor 1 ; ADP-Ribosylation Factor 1 - analysis ; ADP-Ribosylation Factor 1 - metabolism ; ALPS motif ; Amino Acid Motifs ; Binding sites ; Biochemistry, Molecular Biology ; Coat Protein Complex I ; Coat Protein Complex I - analysis ; Coat Protein Complex I - metabolism ; Diffusion ; Golgi Apparatus ; Golgi Apparatus - metabolism ; GTPase-Activating Proteins ; GTPase-Activating Proteins - analysis ; GTPase-Activating Proteins - metabolism ; Life Sciences ; Lipids ; membrane curvature ; Membrane Lipids ; Membrane Lipids - analysis ; Membrane Lipids - metabolism ; membrane tube ; Membranes ; Molecular biology ; Optical Tweezers ; Protein Binding ; Proteins ; Unilamellar Liposomes ; Unilamellar Liposomes - analysis ; Unilamellar Liposomes - metabolism
É parte de: The EMBO journal, 2010-01, Vol.29 (2), p.292-303
Notas: Supplementary InformationReview Process File
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ArticleID:EMBJ2009341
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content type line 23
PMCID: PMC2824456
These authors contributed equally to this work
Descrição: ArfGAP1, which promotes GTP hydrolysis on the small G protein Arf1 on Golgi membranes, interacts preferentially with positively curved membranes through its amphipathic lipid packing sensor (ALPS) motifs. This should influence the distribution of Arf1‐GTP when flat and curved regions coexist on a continuous membrane, notably during COPI vesicle budding. To test this, we pulled tubes from giant vesicles using molecular motors or optical tweezers. Arf1‐GTP distributed on the giant vesicles and on the tubes, whereas ArfGAP1 bound exclusively to the tubes. Decreasing the tube radius revealed a threshold of R≈35 nm for the binding of ArfGAP1 ALPS motifs. Mixing catalytic amounts of ArfGAP1 with Arf1‐GTP induced a smooth Arf1 gradient along the tube. This reflects that Arf1 molecules leaving the tube on GTP hydrolysis are replaced by new Arf1‐GTP molecules diffusing from the giant vesicle. The characteristic length of the gradient is two orders of magnitude larger than a COPI bud, suggesting that Arf1‐GTP diffusion can readily compensate for the localized loss of Arf1 during budding and contribute to the stability of the coat until fission.
Editor: Chichester, UK: John Wiley & Sons, Ltd
Idioma: Inglês

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ArfGAP1 generates an Arf1 gradient on continuous lipid membranes displaying flat and curved regions

Ambroggio, Ernesto ; Sorre, Benoît ; Bassereau, Patricia ; Goud, Bruno ; Manneville, Jean-Baptiste ; Antonny, Bruno

Chichester, UK: John Wiley & Sons, Ltd

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