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The single GUV method for revealing the functions of antimicrobial, pore-forming toxin, and cell-penetrating peptides or proteins

Islam, Md. Zahidul ; Alam, Jahangir Md ; Tamba, Yukihiro ; Karal, Mohammad Abu Sayem ; Yamazaki, Masahito

Physical chemistry chemical physics : PCCP, 2014-01, Vol.16 (3), p.15752-15767 [Periódico revisado por pares]

England

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  • Título:
    The single GUV method for revealing the functions of antimicrobial, pore-forming toxin, and cell-penetrating peptides or proteins
  • Autor: Islam, Md. Zahidul ; Alam, Jahangir Md ; Tamba, Yukihiro ; Karal, Mohammad Abu Sayem ; Yamazaki, Masahito
  • Assuntos: Animals ; Anti-Infective Agents - pharmacology ; Antiinfectives and antibacterials ; Lipids ; Membranes ; Peptides ; Peptides - pharmacology ; Pore formation ; Pore Forming Cytotoxic Proteins - pharmacology ; Rate constants ; Toxins ; Vesicles
  • É parte de: Physical chemistry chemical physics : PCCP, 2014-01, Vol.16 (3), p.15752-15767
  • Notas: Jahangir Md. Alam is a post-doctoral fellow in the Research Institute of Electronics, Shizuoka University, Japan. Concurrently he is a lecturer at the Faculty of Applied Science and Technology, Islamic University, Bangladesh. He received his PhD from Shizuoka University in 2013. His research interest is to reveal the mechanism of the pore formation induced by pore-forming toxin and antimicrobial peptides.
    Masahito Yamazaki is currently a professor at the Research Institute of Electronics, Shizuoka University, Japan. He is also a professor at the Dept. Physics, Graduate School of Science, and at the Dept. Integrated Bioscience, Graduate School of Science and Technology, Shizuoka University. He received his PhD from Kyoto University. His research interest is to develop new imaging methods for investigation of structures, functions, and dynamics of biomembranes and cells, and to reveal their elementary processes and mechanisms.
    Yukihiro Tamba is currently an associate professor of physics at the Suzuka National College of Technology, Japan. He received his PhD from Shizuoka University, Japan, in 2005. His research interest is to develop a new imaging system for the single GUV method and also to reveal the mechanism of the interaction of flavonoids with lipid membranes.
    Mohammad Abu Sayem Karal is currently a PhD student at the Graduate School of Science and Technology, Shizuoka University, Japan. He is an Assistant Professor, Department of Physics, Bangladesh University of Engineering and Technology (BUET), Bangladesh. He received his MS degree in 2002 from the University of Dhaka, Bangladesh. His current research interest is peptide- and tension-induced pore formation of giant liposomes and understanding their biophysical phenomena.
    Md. Zahidul Islam is currently a PhD student at the Graduate School of Science and Technology, Shizuoka University, Japan. He received his MS degree in 2003 from Khulna University, Bangladesh. From 2003 to 2012, he worked in pharmaceutical companies. His research interest is to reveal the mechanism or mode of action of cell penetrating peptides on lipid bilayers by the single GUV method.
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  • Descrição: We recently developed the single giant unilamellar vesicle (GUV) method for investigating the functions and dynamics of biomembranes. The single GUV method can provide detailed information on the elementary processes of physiological phenomena in biomembranes, such as their rate constants. Here we describe the process of pore formation induced by the antimicrobial peptide (AMP), magainin 2, and the pore-forming toxin (PFT), lysenin, as revealed by the single GUV method. We obtained the rate constants of several elementary steps, such as peptide/protein-induced pore formation in lipid membranes and the membrane permeation of fluorescent probes through the pores. Information on the entry of the cell-penetrating peptide (CPP), transportan 10 (TP10), into a single GUV and its induced pore formation in lipid membranes was also obtained. We compare the single GUV method with other methods for investigating the interaction of peptides/proteins with lipid membranes ( i.e. , the large unilamellar vesicle (LUV) suspension method, the GUV suspension method, and single channel recording), and discuss the pros and cons of the single GUV method. On the basis of these data, we discuss the advantages of the single GUV method. The single GUV method provides detailed information on the elementary processes of peptide/protein-induced pore formation in lipid membranes and the entry of peptides into a GUV; specifically, the GUV method provides the rate constants of these processes.
  • Editor: England
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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