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A Chain‐Elongated Oligophenylenevinylene Electrolyte Increases Microbial Membrane Stability

Zhou, Cheng ; Chia, Geraldine W. N. ; Ho, James C. S. ; Moreland, Alex S. ; Seviour, Thomas ; Liedberg, Bo ; Parikh, Atul N. ; Kjelleberg, Staffan ; Hinks, Jamie ; Bazan, Guillermo C.

Advanced materials (Weinheim), 2019-05, Vol.31 (18), p.e1808021-n/a [Periódico revisado por pares]

Germany: Wiley Subscription Services, Inc

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Título:
A Chain‐Elongated Oligophenylenevinylene Electrolyte Increases Microbial Membrane Stability
Autor: Zhou, Cheng ; Chia, Geraldine W. N. ; Ho, James C. S. ; Moreland, Alex S. ; Seviour, Thomas ; Liedberg, Bo ; Parikh, Atul N. ; Kjelleberg, Staffan ; Hinks, Jamie ; Bazan, Guillermo C.
Assuntos: biofuels ; Butanol ; butanol tolerance ; Butanols - toxicity ; Cell Wall - drug effects ; Cell Wall - metabolism ; Chains ; conjugated oligoelectrolytes ; E coli ; Electrolytes - chemistry ; Electrolytes - pharmacology ; Electrolytic cells ; Elongation ; Escherichia coli - drug effects ; Escherichia coli - metabolism ; Fluorescence ; Lipids ; Lipopolysaccharides - chemistry ; Materials science ; membrane integrity ; membrane stability ; Membranes ; Microbial Sensitivity Tests ; Microorganisms ; Microscopy, Confocal ; Stability ; Topology ; Toxicity ; Vinyl Compounds - chemistry
É parte de: Advanced materials (Weinheim), 2019-05, Vol.31 (18), p.e1808021-n/a
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: A novel conjugated oligoelectrolyte (COE) material, named S6, is designed to have a lipid‐bilayer stabilizing topology afforded by an extended oligophenylenevinylene backbone. S6 intercalates biological membranes acting as a hydrophobic support for glycerophospholipid acyl chains. Indeed, Escherichia coli treated with S6 exhibits a twofold improvement in butanol tolerance, a relevant feature to achieve within the general context of modifying microorganisms used in biofuel production. Filamentous growth, a morphological stress response to butanol toxicity in E. coli, is observed in untreated cells after incubation with 0.9% butanol (v/v), but is mitigated by S6 treatment. Real‐time fluorescence imaging using giant unilamellar vesicles reveals the extent to which S6 counters membrane instability. Moreover, S6 also reduces butanol‐induced lipopolysaccharide release from the outer membrane to further maintain cell integrity. These findings highlight a deliberate effort in the molecular design of a chain‐elongated COE to stabilize microbial membranes against environmental challenges. A novel membrane‐intercalating phenylenevinylene oligoelectrolyte S6, comprising a long conjugated backbone, is designed, which can be used to attain a twofold improvement in the butanol tolerance of Escherichia coli. Biophysical experiments based on giant unilamellar vesicles indicate that S6 counters membrane instability caused by butanol. This study demonstrates the power of materials design to achieve simple‐to‐apply methods that aid in inproving strategic properties of microbes.
Editor: Germany: Wiley Subscription Services, Inc
Idioma: Inglês

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A Chain‐Elongated Oligophenylenevinylene Electrolyte Increases Microbial Membrane Stability

Zhou, Cheng ; Chia, Geraldine W. N. ; Ho, James C. S. ; Moreland, Alex S. ; Seviour, Thomas ; Liedberg, Bo ; Parikh, Atul N. ; Kjelleberg, Staffan ; Hinks, Jamie ; Bazan, Guillermo C.

Germany: Wiley Subscription Services, Inc

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