Idioma:

Genesis of fecal floatation is causally linked to gut microbial colonization in mice

Aalam, Syed Mohammed Musheer ; Crasta, Daphne Norma ; Roy, Pooja ; Miller, 2nd, A Lee ; Gamb, Scott I ; Johnson, Stephen ; Till, Lisa M ; Chen, Jun ; Kashyap, Purna ; Kannan, Nagarajan

Scientific reports, 2022-10, Vol.12 (1), p.18109-18109, Article 18109 [Periódico revisado por pares]

England: Nature Publishing Group

Texto completo disponível

Citações Citado por

Enviar para

Título:
Genesis of fecal floatation is causally linked to gut microbial colonization in mice
Autor: Aalam, Syed Mohammed Musheer ; Crasta, Daphne Norma ; Roy, Pooja ; Miller, 2nd, A Lee ; Gamb, Scott I ; Johnson, Stephen ; Till, Lisa M ; Chen, Jun ; Kashyap, Purna ; Kannan, Nagarajan
Assuntos: Animal models ; Animals ; Bacteria - genetics ; Biotransformation ; Buoyancy ; Colonization ; Digestive system ; Digestive tract ; Discordance ; Fecal Microbiota Transplantation ; Feces ; Feces - microbiology ; Flow cytometry ; Gastrointestinal Microbiome ; Germfree ; Humans ; Metagenomics ; Mice ; Microbiota ; Scanning electron microscopy
É parte de: Scientific reports, 2022-10, Vol.12 (1), p.18109-18109, Article 18109
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: The origin of fecal floatation phenomenon remains poorly understood. Following our serendipitous discovery of differences in buoyancy of feces from germ-free and conventional mice, we characterized microbial and physical properties of feces from germ-free and gut-colonized (conventional and conventionalized) mice. The gut-colonization associated differences were assessed in feces using DNA, bacterial-PCR, scanning electron microscopy, FACS, thermogravimetry and pycnometry. Based on the differences in buoyancy of feces, we developed levô in fimo test (LIFT) to distinguish sinking feces (sinkers) of germ-free mice from floating feces (floaters) of gut-colonized mice. By simultaneous tracking of microbiota densities and gut colonization kinetics in fecal transplanted mice, we provide first direct evidence of causal relationship between gut microbial colonization and fecal floatation. Rare discordance in LIFT and microbiota density indicated that enrichment of gasogenic gut colonizers may be necessary for fecal floatation. Finally, fecal metagenomics analysis of 'floaters' from conventional and syngeneic fecal transplanted mice identified colonization of > 10 gasogenic bacterial species including highly prevalent B. ovatus, an anaerobic commensal bacteria linked with flatulence and intestinal bowel diseases. The findings reported here will improve our understanding of food microbial biotransformation and gut microbial regulators of fecal floatation in human health and disease.
Editor: England: Nature Publishing Group
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Anterior Resultado 6 Avançar Ir para próxima página

Realização: Logos de Redes Sociais:

Genesis of fecal floatation is causally linked to gut microbial colonization in mice

Aalam, Syed Mohammed Musheer ; Crasta, Daphne Norma ; Roy, Pooja ; Miller, 2nd, A Lee ; Gamb, Scott I ; Johnson, Stephen ; Till, Lisa M ; Chen, Jun ; Kashyap, Purna ; Kannan, Nagarajan

England: Nature Publishing Group

Buscando em bases de dados remotas. Favor aguardar.