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Resistance to Linezolid Is Mediated by the cfr Gene in the First Report of an Outbreak of Linezolid-Resistant Staphylococcus aureus

Morales, Gracia ; Picazo, Juan J. ; Baos, Elvira ; Candel, Francisco J. ; Arribi, Ana ; Peláez, Beatriz ; Andrade, Raquel ; de la Torre, María-Ángeles ; Fereres, José ; Sánchez-García, Miguel

Clinical infectious diseases, 2010-03, Vol.50 (6), p.821-825 [Periódico revisado por pares]

Oxford: The University of Chicago Press

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Título:
Resistance to Linezolid Is Mediated by the cfr Gene in the First Report of an Outbreak of Linezolid-Resistant Staphylococcus aureus
Autor: Morales, Gracia ; Picazo, Juan J. ; Baos, Elvira ; Candel, Francisco J. ; Arribi, Ana ; Peláez, Beatriz ; Andrade, Raquel ; de la Torre, María-Ángeles ; Fereres, José ; Sánchez-García, Miguel
Assuntos: Acetamides - pharmacology ; Anti-Bacterial Agents - pharmacology ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; ARTICLES AND COMMENTARIES ; Bacterial diseases ; Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Biological and medical sciences ; Disease Outbreaks ; DNA Fingerprinting ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Drug resistance ; Drug Resistance, Bacterial ; Electrophoresis ; Electrophoresis, Gel, Pulsed-Field ; Epidemics ; Gels ; Genes ; Genetic mutation ; Genotype ; Hospital units ; Human bacterial diseases ; Humans ; Infectious diseases ; Intensive care ; Intensive care units ; Linezolid ; Medical sciences ; Methicillin resistant staphylococcus aureus ; Methicillin-Resistant Staphylococcus aureus - classification ; Methicillin-Resistant Staphylococcus aureus - drug effects ; Methicillin-Resistant Staphylococcus aureus - genetics ; Methicillin-Resistant Staphylococcus aureus - isolation & purification ; Microbial Sensitivity Tests ; Oxazolidinones - pharmacology ; Pharmacology. Drug treatments ; Polymerase Chain Reaction ; Resistance mechanisms ; Ribonucleic acid ; RNA ; RNA, Ribosomal, 23S - genetics ; Sequence Analysis, DNA ; Staphylococcal Infections - epidemiology ; Staphylococcal Infections - microbiology ; Staphylococcal infections, streptococcal infections, pneumococcal infections ; Staphylococcus ; Staphylococcus aureus ; Staphylococcus infections
É parte de: Clinical infectious diseases, 2010-03, Vol.50 (6), p.821-825
Notas: ark:/67375/HXZ-F8KJH9XW-9
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Descrição: Background. From April through June 2008, we identified 12 patients in the intensive care unit and 3 patients on other wards infected with methicillin-resistant Staphylococcus aureus that was also resistant to linezolid. We investigated the mechanism of resistance —point mutations in domain V of 23S ribosomal RNA (rRNA) or presence of the cfr gene —involved in the outbreak. Methods. Strains for the study were obtained in the intensive care unit and other wards. Minimal inhibitory concentrations were determined using automated methods, the E-test, or dilution in Mueller-Hinton agar in accordance with Clinical and Laboratory Standards Institute guidelines. Strains were genotyped using pulsed-field gel electrophoresis and were sequenced to determine the presence of point mutations in 23S rRNA. The presence of the cfr gene was determined by specific polymerase chain reaction. Results. The minimal inhibitory concentrations of linezolid ranged from 16 mg/L to 32 mg/L, and all the strains were susceptible to tigecycline, vancomycin, and daptomycin. Typing of strains sequentially isolated by pulsed-field gel electrophoresis showed that each patient carried only 1 clonal type of linezolid-resistant, methicillin-resistant S. aureus as detected by sequential isolations. The presence of the cfr gene was confirmed in all the isolates. Furthermore, sequencing of domain V of 23S rRNA showed that the most common mechanism of linezolid resistance reported to date, mutation G2576T, was not detected in any of the strains analyzed. Conclusions. We report the presence of the cfr gene underlying the resistance mechanism involved in a clinical outbreak of linezolid-resistant S. aureus.
Editor: Oxford: The University of Chicago Press
Idioma: Inglês

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Resistance to Linezolid Is Mediated by the cfr Gene in the First Report of an Outbreak of Linezolid-Resistant Staphylococcus aureus

Morales, Gracia ; Picazo, Juan J. ; Baos, Elvira ; Candel, Francisco J. ; Arribi, Ana ; Peláez, Beatriz ; Andrade, Raquel ; de la Torre, María-Ángeles ; Fereres, José ; Sánchez-García, Miguel

Oxford: The University of Chicago Press

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