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Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts

Mendes, Adriano A ; de Castro, Heizir F ; de S. Rodrigues, Dasciana ; Adriano, Wellington S ; Tardioli, Paulo W ; Mammarella, Enrique J ; de C. Giordano, Roberto ; de L. C. Giordano, Raquel

Journal of industrial microbiology & biotechnology, 2011-08, Vol.38 (8), p.1055-1066 [Periódico revisado por pares]

Berlin/Heidelberg: Springer-Verlag

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Título:
Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts
Autor: Mendes, Adriano A ; de Castro, Heizir F ; de S. Rodrigues, Dasciana ; Adriano, Wellington S ; Tardioli, Paulo W ; Mammarella, Enrique J ; de C. Giordano, Roberto ; de L. C. Giordano, Raquel
Assuntos: Alginates - chemistry ; Ascomycota - metabolism ; Biocatalysts ; Biochemistry ; Biodiesel fuels ; Biofuel production ; Biofuels ; Bioinformatics ; Biological and medical sciences ; Biomedical and Life Sciences ; Biopolymers ; Biosynthesis ; Biotechnology ; Carrageenan - chemistry ; Carrageenan - metabolism ; Catalysis ; Catalysts ; Chemical engineering ; chitosan ; Chitosan - chemistry ; Electrolytes - chemistry ; Energy ; enzyme activity ; Enzymes ; Enzymes, Immobilized - chemistry ; Enzymes, Immobilized - metabolism ; Epoxy Compounds - chemistry ; Esterification ; Fundamental and applied biological sciences. Psychology ; gelatin ; General aspects ; Genetic Engineering ; Glucuronic Acid - chemistry ; Glutaral - chemistry ; Half-Life ; Hexuronic Acids - chemistry ; hybrids ; hydrocolloids ; Hydrogels ; Hydrogels - chemistry ; Hydrogen-Ion Concentration ; Hydrolysis ; hydrophobicity ; Immobilization techniques ; immobilized enzymes ; industrial applications ; Industrial applications and implications. Economical aspects ; Inorganic Chemistry ; Life Sciences ; Lipase - chemistry ; Lipase - metabolism ; Methods. Procedures. Technologies ; Microbiology ; Original Paper ; palm oils ; Polyvinyl alcohol ; Polyvinyl Alcohol - chemistry ; Propanols - chemistry ; Proteins ; Studies ; sulfonic acid ; thermal stability ; Thermomyces lanuginosus ; transesterification ; triacylglycerol lipase ; Triglycerides ; vegetable oil ; Vegetable oils
É parte de: Journal of industrial microbiology & biotechnology, 2011-08, Vol.38 (8), p.1055-1066
Notas: http://dx.doi.org/10.1007/s10295-010-0880-9
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SourceType-Scholarly Journals-1
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Descrição: This work aimed at the production of stabilized derivatives of Thermomyces lanuginosus lipase (TLL) by multipoint covalent immobilization of the enzyme on chitosan-based matrices. The resulting biocatalysts were tested for synthesis of biodiesel by ethanolysis of palm oil. Different hydrogels were prepared: chitosan alone and in polyelectrolyte complexes (PEC) with κ-carrageenan, gelatin, alginate, and polyvinyl alcohol (PVA). The obtained supports were chemically modified with 2,4,6-trinitrobenzene sulfonic acid (TNBS) to increase support hydrophobicity, followed by activation with different agents such as glycidol (GLY), epichlorohydrin (EPI), and glutaraldehyde (GLU). The chitosan-alginate hydrogel, chemically modified with TNBS, provided derivatives with higher apparent hydrolytic activity (HAapp) and thermal stability, being up to 45-fold more stable than soluble lipase. The maximum load of immobilized enzyme was 17.5 mg g−1 of gel for GLU, 7.76 mg g−1 of gel for GLY, and 7.65 mg g−1 of gel for EPI derivatives, the latter presenting the maximum apparent hydrolytic activity (364.8 IU g−1 of gel). The three derivatives catalyzed conversion of palm oil to biodiesel, but chitosan-alginate-TNBS activated via GLY and EPI led to higher recovered activities of the enzyme. Thus, this is a more attractive option for both hydrolysis and transesterification of vegetable oils using immobilized TLL, although industrial application of this biocatalyst still demands further improvements in its half-life to make the enzymatic process economically attractive.
Editor: Berlin/Heidelberg: Springer-Verlag
Idioma: Inglês

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Multipoint covalent immobilization of lipase on chitosan hybrid hydrogels: influence of the polyelectrolyte complex type and chemical modification on the catalytic properties of the biocatalysts

Mendes, Adriano A ; de Castro, Heizir F ; de S. Rodrigues, Dasciana ; Adriano, Wellington S ; Tardioli, Paulo W ; Mammarella, Enrique J ; de C. Giordano, Roberto ; de L. C. Giordano, Raquel

Berlin/Heidelberg: Springer-Verlag

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