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Synthesis and Spectroscopic Characterization of Fluorophore-Labeled Oligospiroketal Rods

Techen, Anne ; Czapla, Sylvia ; Möllnitz, Kristian ; Budach, Dennis ; Wessig, Pablo ; Kumke, Michael U.

Helvetica chimica acta, 2013-11, Vol.96 (11), p.2046-2067 [Periódico revisado por pares]

Zürich: WILEY-VCH Verlag

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  • Título:
    Synthesis and Spectroscopic Characterization of Fluorophore-Labeled Oligospiroketal Rods
  • Autor: Techen, Anne ; Czapla, Sylvia ; Möllnitz, Kristian ; Budach, Dennis ; Wessig, Pablo ; Kumke, Michael U.
  • Assuntos: Carboxyfluorescein ; Carboxyrhodamine ; Fluorescence ; Förster resonance energy transfer (FRET) ; Molecular rods ; Perylene ; Pyrene
  • É parte de: Helvetica chimica acta, 2013-11, Vol.96 (11), p.2046-2067
  • Notas: ArticleID:HLCA201200616
    istex:342904B2A3431E6970E57CB7057063B9A2758549
    ark:/67375/WNG-3R2K5BK0-Z
  • Descrição: Fluorescence probes consisting of well‐established fluorophores in combination with rigid molecular rods based on spirane‐type structures were investigated with respect to their fluorescence properties under different solvent conditions. The attachment of the dyes was accomplished by 1,3‐dipolar cycloaddition between alkynes and azides (‘click’ reaction) and is a prime example for a novel class of sensor constructs. Especially, the attachment of two (different) fluorophores on opposite sides of the molecular rods paves the way to new sensor systems with less bulky (compared to the conventional DNA‐ or protein‐based concepts), nevertheless rigid spacer constructs, e.g., for FRET‐based sensing applications. A detailed photophysical characterization was performed in MeOH (and in basic H2O/MeOH mixtures) for i) rod constructs containing carboxyfluorescein, ii) rod constructs containing carboxyrhodamine, iii) rod constructs containing both carboxyfluorescein and carboxyrhodamine, and iv) rod constructs containing both pyrene and perylene parts. For each dye (pair), two rod lengths with different numbers of spirane units were synthesized and investigated. The rod constructs were characterized in ensemble as well as single‐molecule fluorescence experiments with respect to i) specific roddye and ii) dyedye interactions. In addition to MeOH and MeOH/NaOH, the rod constructs were also investigated in micellar systems, which were chosen as a simplified model for membranes.
  • Editor: Zürich: WILEY-VCH Verlag
  • Idioma: Inglês;Francês;Alemão;Italiano
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