skip to main content
Idioma:

Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms

Ganguly, Chhandosee ; Rostami, Saadi ; Long, Kole ; Aribam, Swarmistha Devi ; Rajan, Rakhi

The Journal of biological chemistry, 2024-06, Vol.300 (6), p.107295, Article 107295 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por
  • Título:
    Unity among the diverse RNA-guided CRISPR-Cas interference mechanisms
  • Autor: Ganguly, Chhandosee ; Rostami, Saadi ; Long, Kole ; Aribam, Swarmistha Devi ; Rajan, Rakhi
  • Assuntos: Cas10 ; Cas12 ; Cas13 ; Cas3 ; Cas9 ; Cascade ; CasDinG ; CRISPR-cas ; crRNA ; RNA binding protein
  • É parte de: The Journal of biological chemistry, 2024-06, Vol.300 (6), p.107295, Article 107295
  • Notas: ObjectType-Article-1
    SourceType-Scholarly Journals-1
    ObjectType-Feature-2
    ObjectType-Review-3
    content type line 23
  • Descrição: CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated) systems are adaptive immune systems that protect bacteria and archaea from invading mobile genetic elements (MGEs). The Cas protein-CRISPR RNA (crRNA) complex uses complementarity of the crRNA “guide” region to specifically recognize the invader genome. CRISPR effectors that perform targeted destruction of the foreign genome have emerged independently as multi-subunit protein complexes (Class 1 systems) and as single multi-domain proteins (Class 2). These different CRISPR-Cas systems can cleave RNA, DNA, and protein in an RNA-guided manner to eliminate the invader, and in some cases, they initiate programmed cell death/dormancy. The versatile mechanisms of the different CRISPR-Cas systems to target and destroy nucleic acids have been adapted to develop various programmable-RNA-guided tools and have revolutionized the development of fast, accurate, and accessible genomic applications. In this review, we present the structure and interference mechanisms of different CRISPR-Cas systems and an analysis of their unified features. The three types of Class 1 systems (I, III, and IV) have a conserved right-handed helical filamentous structure that provides a backbone for sequence-specific targeting while using unique proteins with distinct mechanisms to destroy the invader. Similarly, all three Class 2 types (II, V, and VI) have a bilobed architecture that binds the RNA-DNA/RNA hybrid and uses different nuclease domains to cleave invading MGEs. Additionally, we highlight the mechanistic similarities of CRISPR-Cas enzymes with other RNA-cleaving enzymes and briefly present the evolutionary routes of the different CRISPR-Cas systems.
  • Editor: United States: Elsevier Inc
  • Idioma: Inglês
Links
Voltar para lista de resultados
Ir para página anteriorAnterior Resultado  2 AvançarIr para próxima página

  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

Buscando em bases de dados remotas. Favor aguardar.