Idioma:

Concentration-dependent Effect of Sodium Hypochlorite on Stem Cells of Apical Papilla Survival and Differentiation

Martin, David E., DDS ; De Almeida, Jose Flavio A., DDS, PhD ; Henry, Michael A., DDS, PhD ; Khaing, Zin Z., PhD ; Schmidt, Christine E., PhD ; Teixeira, Fabricio B., DDS, MS, PhD ; Diogenes, Anibal, DDS, PhD

Journal of endodontics, 2014, Vol.40 (1), p.51-55 [Periódico revisado por pares]

United States: Elsevier Inc

Texto completo disponível

Citações Citado por

Enviar para

Título:
Concentration-dependent Effect of Sodium Hypochlorite on Stem Cells of Apical Papilla Survival and Differentiation
Autor: Martin, David E., DDS ; De Almeida, Jose Flavio A., DDS, PhD ; Henry, Michael A., DDS, PhD ; Khaing, Zin Z., PhD ; Schmidt, Christine E., PhD ; Teixeira, Fabricio B., DDS, MS, PhD ; Diogenes, Anibal, DDS, PhD
Assuntos: Cell Culture Techniques ; Cell Differentiation - drug effects ; Cell Survival - drug effects ; Cells, Cultured ; Dental Papilla - cytology ; Dentin - cytology ; Dentin conditioning ; dentin sialophosphoprotein ; Dentistry ; Dose-Response Relationship, Drug ; Edetic Acid - therapeutic use ; EDTA ; Endocrinology & Metabolism ; Extracellular Matrix Proteins - analysis ; Extracellular Matrix Proteins - drug effects ; gene expression ; Humans ; Hyaluronic Acid - chemistry ; irrigant ; Luminescent Agents ; Odontoblasts - drug effects ; Phosphoproteins - analysis ; Phosphoproteins - drug effects ; regenerative endodontics ; Root Canal Irrigants - administration & dosage ; Root Canal Irrigants - pharmacology ; Sialoglycoproteins - analysis ; Sialoglycoproteins - drug effects ; sodium hypochlorite ; Sodium Hypochlorite - administration & dosage ; Sodium Hypochlorite - pharmacology ; Stem Cells - drug effects ; stem cells of apical papilla ; survival ; Tissue Engineering - methods ; Tissue Scaffolds - chemistry ; Tooth Apex - cytology ; viability
É parte de: Journal of endodontics, 2014, Vol.40 (1), p.51-55
Notas: ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
Descrição: Abstract Introduction Intracanal disinfection is a crucial step in regenerative endodontic procedures. Most published cases suggest the use of sodium hypochlorite (NaOCl) as the primary irrigant. However, the effect of clinically used concentrations of NaOCl on the survival and differentiation of stem cells is largely unknown. In this study, we tested the effect of various concentrations of NaOCl on the stem cells of the apical papilla (SCAPs) survival and dentin sialophosphoprotein (DSPP) expression. Methods Standardized root canals were created in extracted human teeth and irrigated with NaOCl (0.5%, 1.5%, 3%, or 6%) followed by 17% EDTA or sterile saline. SCAPs in a hyaluronic acid–based scaffold were seeded into the canals and cultured for 7 days. Next, viable cells were quantified using a luminescence assay, and DSPP expression was evaluated using quantitative real-time polymerase chain reaction. Results There was a significant reduction in survival and DSPP expression in the group treated with 6% NaOCl compared with the untreated control group. Comparable survival was observed in the groups treated with the lower concentrations of NaOCl, but greater DSPP expression was observed in the 1.5% NaOCl group. In addition, 17% EDTA resulted in increased survival and DSPP expression partially reversing the deleterious effects of NaOCl. Conclusions Collectively, the results suggest that dentin conditioning with high concentrations of NaOCl has a profound negative effect on the survival and differentiation of SCAPs. However, this effect can be prevented with the use of 1.5% NaOCl followed by 17% EDTA. The inclusion of this irrigation regimen might be beneficial in regenerative endodontic procedures.
Editor: United States: Elsevier Inc
Idioma: Inglês

Links

View this record in MEDLINE/PubMed

Voltar para lista de resultados

Realização: Logos de Redes Sociais:

Concentration-dependent Effect of Sodium Hypochlorite on Stem Cells of Apical Papilla Survival and Differentiation

Martin, David E., DDS ; De Almeida, Jose Flavio A., DDS, PhD ; Henry, Michael A., DDS, PhD ; Khaing, Zin Z., PhD ; Schmidt, Christine E., PhD ; Teixeira, Fabricio B., DDS, MS, PhD ; Diogenes, Anibal, DDS, PhD

United States: Elsevier Inc

Buscando em bases de dados remotas. Favor aguardar.