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Ultra‐high‐performance liquid chromatography combined with electrospray ionization quadrupole time‐of‐flight mass spectrometry and molecular networking analysis to investigate the chemodiversity of bioactive extracts of Annona jahnii Saff. fungi from the Brazilian Amazon

Xavier, Luciana Araújo ; Nascimento Odilair, Letícia Maciel ; Sousa, Gilmara Prado ; Souza, Edineide Cristina A. ; Pilau, Eduardo Jorge ; Porto, Carla ; Souza, Antônia Queiroz Lima ; Souza, Afonso Duarte Leão ; Flach, Adriana ; Costa, Luiz Antonio M. A.

Rapid communications in mass spectrometry, 2022-10, Vol.36 (19), p.e9356-n/a [Periódico revisado por pares]

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  • Título:
    Ultra‐high‐performance liquid chromatography combined with electrospray ionization quadrupole time‐of‐flight mass spectrometry and molecular networking analysis to investigate the chemodiversity of bioactive extracts of Annona jahnii Saff. fungi from the Brazilian Amazon
  • Autor: Xavier, Luciana Araújo ; Nascimento Odilair, Letícia Maciel ; Sousa, Gilmara Prado ; Souza, Edineide Cristina A. ; Pilau, Eduardo Jorge ; Porto, Carla ; Souza, Antônia Queiroz Lima ; Souza, Afonso Duarte Leão ; Flach, Adriana ; Costa, Luiz Antonio M. A.
  • Assuntos: Anticancer properties ; Antiinfectives and antibacterials ; Antimicrobial agents ; Antioxidants ; Biological activity ; Biological properties ; Chromatography ; Coumarin ; E coli ; Electrospraying ; Ethyl acetate ; Feature extraction ; Free radicals ; Fungi ; Ionization ; Ions ; Liquid chromatography ; Mass spectrometry ; Metabolites ; Natural products ; Quadrupoles ; Scientific imaging ; Spectroscopy
  • É parte de: Rapid communications in mass spectrometry, 2022-10, Vol.36 (19), p.e9356-n/a
  • Notas: All the authors contributed equally to this work.
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  • Descrição: Rationale Annona species are of interest for the isolation of bioactive molecules; however, studies of Annona jahnii Saff. are limited. The exploration of bioactive metabolites of endophytes isolated from this species is unprecedented and allows the preservation of the host plant, in addition to enabling the discovery of compounds with promising biological activities. Methods Ethyl acetate extracts from the cultured media of five fungi were obtained. The antioxidant capacity of the extracts was measured using the 1,1‐diphenyl‐2‐picrylhydrazyl free radical method. Antimicrobial activity was determined using the microdilution method in broth in 96‐well plates. The exploration of the metabolic profile of the extracts and dereplication of the compounds were performed using ultra‐high‐performance liquid chromatography/electrospray ionization/tandem mass spectrometry (UHPLC/ESI‐MS/MS) combined with analysis using molecular networking (MN). Results A total of 1818 MS features were detected in the five selected extracts, of which 39 compounds were putatively identified. The secondary metabolites with the highest abundance were alkaloids, naphthopyrons, and cytochalasins. Other secondary metabolites include fumonisins, coumarin, and a meroterpenoid. Most of these compounds are related to specific biological properties such as antioxidant, anti‐inflammatory, antimicrobial, antiviral, and antitumor activities. Extracts F398 and F403 showed inhibitory activity of the four pathogens tested. Extracts F475 and F506 did not inhibit the growth of Staphylococcus aureus, and F407 did not inhibit the growth of Escherichia coli in addition to having potent antioxidant activity, with IC50 values of 10 μg/mL or less. Conclusions The use of UHPLC/ESI‐MS/MS data combined with MN proved useful in the dereplication of bioactive molecules of complex extracts that are still unexplored. These initial investigations should significantly assist in further research and increase the efficiency and speed in the discovery of new sources of secondary metabolites and new natural products.
  • Editor: Bognor Regis: Wiley Subscription Services, Inc
  • Idioma: Inglês
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  • Realização: ABCD-USP USP   Logos de Redes Sociais: Freepik

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